Objective. The purpose of this study is to identify novel biomarkers for the prognosis of Ewing’s sarcoma based on bioinformatics analysis. Methods. The GSE63157 and GSE17679 datasets contain patient and healthy control microarray data that were downloaded from the Gene Expression Omnibus (GEO) database and analyzed through R language software to obtain differentially expressed genes (DEGs). Firstly, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) functional enrichment, protein-protein interaction (PPI) networks, and Cytoscape Molecular Complex Detection (MCODE) plug-in were then used to compute the highest scores of the module. After survival analysis, the hub genes were lastly obtained from the two module genes. Results. A total of 1181 DEGs were identified from the two GSEs. Through MCODE and survival analysis, we obtain 53 DEGs from the module and 29 overall survival- (OS-) related genes. ZBTB16 was the only downregulated gene after Venn diagrams. Survival analysis indicates that there was a significant correlation between the high expression of ZBTB16 and the OS of Ewing’s sarcoma (ES), and the low expression group had an unfavorable OS when compared to the high expression group. Conclusions. High expression of ZBTB16 may serve as a predictor biomarker of poor prognosis in ES patients.

A discussion is conducted on the problems of ideological and political education, rotation learning in departments, clinical and scientific research capabilities, paper writing, and improvement of the quality of papers encountered by professional master of clinical medicine in the "two-track integration" mode during the standardized training of resident doctors. On that basis, several methods are proposed in the present study (e.g., stressing ideological education for professional masters, coordinating learn of professional skills, enhancing paper writing ability and scientific research ability, organizing a professional team of tutors). On that basis, this study summarizes the problems and challenges exposed in training professional medical students, especially under "two-track integration" mode, seeks to propose a novel idea to serve better for the "two-track integration" educational mode, including provide solutions to help students establish learning goals, to enhance their writing ability, to narrow the bridge between teachers and students, etc. All of which, aims to optimize the training of professional masters of clinical medicine, and give some feasible suggestions for subsequent research. Hopefully, the suggestions and proposal in this study would draw attention and serve better to increase the education quality on professional medical students.

To review on the difficulties encountered by medical bachelor students for their career development after graduation, and to explore potential solutions to their current situation, thus provide them possibilities of making good use of professional training and skills acquired in campus.

Through the reform of the current postgraduate training mode and the way of mentoring and teaching, such as increasing the proportion of scientific research-related courses, raising the threshold for graduation, establishing a team of tutors to provide effective guidance to students, etc. The idea of forming a postgraduate scientific research team is proposed, and students of different majors and degree types are encouraged to participate in scientific research activities as a team, so as to improve the scientific research atmosphere and scientific research enthusiasm of professional master of clinical medicine (hereinafter referred to as "professional master"), in order to achieve the goal of improving the scientific research and innovation ability of "professional master", put forward solutions for the problems encountered in the context of the "Double Tracks in One" policy and the large-scale expansion of postgraduate enrollment, and provide theoretical and theoretical ideas for the cultivation of innovation ability of "professional master". Practical basis.

The effect of clinical practice directly affects the clinical ability and professional quality of graduates, lack of interest in practice, lack of doctor-patient communication skills and conflict with other clinical training doctors are common problems in current practice. This article through to the clinical medical undergraduate interns in practice there is lack of interest, in the process of doctor-patient communication skills and conflict with other clinical training for doctors and other issues were discussed, and through the reform of teaching methods, strengthen the teaching supervision and organize training of doctor-patient communication, in order to improve the clinical interns learning autonomy, strengthen the doctor-patient communication skills, to further improve the quality of medical personnel training to provide help.