INTRODUCTION:The correction of the gingival recession is of esthetical and functional significance, but the tissue regeneration can only be confirmed by a histological examination.AIM:This study aims to make a comparison between the free gingival graft and the autograft.MATERIAL AND METHODS:This study included 24 patients with single and multiple gingival recessions. Twelve patients were treated with a free gingival graft and the other twelve with a micrograft. Six months after the surgical procedure, a micro-punch biopsy of the transplantation area was performed. The tissue was histologically evaluated, graded in 4 categories: immature, mature, fragmented and edematous collagen tissue. The elastic fibres were also examined and graded in three categories: with a normal structure, fragmented rare and fragmented multiplied.RESULTS:Regarding the type of collagen tissue that was present, there was a significant difference between the two groups of patients, with a larger number of patients treated with a micrograft showing a presence of mature tissue, compared to the patients treated with a free gingival graft. A larger number of patients in both of the groups displayed elastic fibres with a rare fragmented structure; 33.3% of the patients showed a normal structure; 50% demonstrated a normal structure.CONCLUSION:The patients treated with a free gingival graft showed a larger presence of fragmented collagen tissue and fragmented elastic fibres, whereas a mature tissue was predominantly present in the surgical area where a Geistlich Mucograft was placed.

BACKGROUND:The success of prosthetic rehabilitation in patients with removable dentures depends on the achievement of the aesthetics, phonetics and most of all, proper use in the mastication process. All the patients that receive removable prostheses need a feeding education program. They must cut the food into smaller pieces, extend the length of time necessary for chewing and place the food upon both the right and left sides of the mouth at once. Bilaterally chewing with dentures will contribute to increased efficiency and denture stability during mastication. Using the anterior teeth for biting, as a result of increased pressure on the anterior ridge may lead to the anterior hyperfunction syndrome.CASE REPORT:The patient requested dental rehabilitation in our clinic for prosthetic dentistry two and a half years ago. We examined him and made therapy plan, for complete removable maxillary denture and partial mandibular denture. Besides our instructions for proper use of dentures and necessity for regular controls, his next visit was after two and a half years. He came with enlarged tuberosity and papillary hyperplasia in the pre-maxillary region. After oral surgery treatment (laser removing of hyperplastic tissue) and a healing period of four weeks, we made indirect relining on the upper denture, re-occlusion and re-articulation achieving weak contacts between the lower natural teeth and upper teeth of the complete denture. The patient was advised not to bite food with his anterior teeth, and avoid chewing very hard food which tends to imprint and displace dentures.CONCLUSION:Anterior hyperfunction syndrome with its high incidence is a disease with the need of interdisciplinary therapy approach. Fast diagnosis, thorough clinical examination using all available diagnostic tools, and choosing the right treatment is very challenging.

Summary Introduction Human organism can be identified through testing and analysis of DNA sequences. The most common source of DNA for analysis is blood, soft tissues, hair, bones and teeth. Teeth represent a tissue of choice for analysis in those cases where there is high degree of degradation of other tissues. Hard tooth structure provides protection and preservation of DNA molecules. The aim was to investigate which group of teeth and dental tissue (pulp or hard dental tissues) has the greatest amount of DNA. Material and method Forty-five extracted teeth were analyzed. In the first examination 30 teeth were divided into the three groups (10 teeth each): first group were incisors, second premolars and third molars. The teeth were measured before and after the procedure of DNA isolation using special scale with precision of 0.02-0.000005ng. The procedure included grinding teeth in a blender and DNA isolation using commercial kits (isolation with magnetic particles). For the second test 15 teeth divided into two groups were used. In the first group isolation of DNA molecules was performed from pulp tissue, and in the second group from hard dental tissues. The quantification of samples was done with Quantifiler® Duo DNA Quantification Kit by Applied Biosystems. Results The greatest amount of DNA was obtained from molars (0.230011ng/μl/g) while the smallest amount of DNA was obtained from incisors and it was 0.06437ng/μl/g. In addition, the amount of DNA isolated from pulp tissue was significantly greater than that from hard dental tissues (pulp of molars obtained quantitatively the largest amount of DNA). Conclusion Main tissue to be used for the isolation of DNA from a tooth is pulp, but in those cases when it is not present (endodontic treatment), hard dental tissues provide sufficient quantity of DNA for identification procedures. The group of teeth that provides the greatest amount of DNA is molars.

Total and partial impaction of the teeth is considered to be a developmental anomaly, that can affect any tooth in both deciduous and permanent dentition, but according to a large number of dental authors it is mostly associated with the mandibular third molars. Its multifactorial etiology, diagnostics, oral surgical approach and techniques can increase the difficulties of this problem which is encountered in the everyday oral surgical practice.The selection of the appropriate oral surgical technique mostly depends on the various positions in which the total or partial impacted mandibular third molar may appear and his correlation with the adjacent anatomical structures, thus leading to different diagnostic and therapeutic problems.This study includes 80 patients, divided in two groups of 40 patients, where one group is diagnosed with a total impaction and the other group with a partial impaction of the mandibular third molars.After a statistical analysis of the obtained data was performed with the help of appropriate world renowned classifications concerning the position of the impacted mandibular third molars, we came to the following results: according to Winter’s classification, the impacted molars where dominantly in a vertical position; Axhausen’s flap design was dominantly a method of choice; buccodistal osteotomy is the most frequently used technique; the impacted molars presented a convergent anatomical configuration of the roots in most of the cases.