Seldimirova O.A., Zaytsev D.Yu., Galin I.R., Kruglova N.N. AbstractThe phenomenon of androgenesis in vitro is widely used in biotechnological investigations of spring wheat. When solving the specific biotechnological problems it is important to know what Seldimirova O.A., Zaytsev D.Yu., Galin I.R., Kruglova N.N. Phytohormonal regulation of in vitro formation of wheat androgenic structures // Сетевой журнал «Научный результат». Серия «Физиология». -Т.2, №1(7), 2016. 4 Серия ФИЗИОЛОГИЯ PHISIOLOGY Series kind of morphogenesis pathway in vitro will result in androgenic plants. This research is devoted to developing a method of phytohormonal regulation of in vitro formation of a certain type of wheat androgenic structures. Using the method of ELISA it was shown that the induction of certain sporophytic morphogenesis pathway in vitro of anther haploid cells -microspores depends on both the content of endogenous auxin IAA in anthers before inoculating them onto induction medium, and the concentration of exogenous auxin 2,4-D in this medium. The obtained data confirms the principle possibility of regulation of ways of getting androgenic regenerants in vitro by selecting the optimal balance of endogenous and exogenous auxins.
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