In recent years, interest in examining the chemical composition andpharmacological properties of apple cider vinegar in synergy with the applicationof natural products in the pharmaceutical, food and cosmetics industry has beengrowing. The apple vinegar shows a wide range of biological activities(antimicrobial, antioxidant, anti-diabetic, anti-inflammatory, antihypertensive,immune-stimulatory, anticancer) and it has been used in traditional medicine for along time. It consists of 8 essential amino acids (phenylalanine, isoleucine, leucine,lysine, methionine, threonine, tryptophan and valine), organic acids, enzymes,minerals (potassium, calcium, phosphorus, copper, grape and boron) and vitamins(provitamin C, vitamin A, E, B1, B2, B6, vitamin P and provitamin Beta-carotene).The aim of the study was to examine the antibacterial activity of traditionallyproduced apple cider vinegar and to determine whether it exhibits bactericidal orbacteriostatic activity. The results of the study confirmed the antibacterial activityof apple cider vinegar produced with the traditional method. Its antibacterialactivity is in the range of 11.33mm to 14mm.

Prirodni ekstrakti pravljeni od svježeg biljnog materijala i biološki aktivna jedinjenja izolovana iz različitih biljnih vrsta koje se već vijekovima koriste u narodnoj medicini mogu predstavljati dragocjene izvore za proizvodnju novih prirodnih konzervanasa i ljekovitih pripravaka. Bijeli ljiljan – Lilium candidum se takođe dugo koristi u tradicionalnoj medicini za liječenje opekotina i promrzlina, upale uha i nosa, kao melem za ispiranje rana i posjekotina. Korijen, listovi i cvjetovi imaju ljekovita svojstva, ali je malo podataka u literaturi o antibakterijskom dejstvu ekstrakta bijelog ljiljana na određene patogene i da se utvrdi opravdanost njegovog korištenja u tradicionalnoj medicini. U radu je ispitana antibakterijska aktivnost ekstrakta bijelog ljiljana na pet referentnih kultura (Escherichia coli WDCM 00090, Listeria monocytogenes WDCM 00020, Salmonella enterica WDCM 00030, Pseudomonas aeruginosa WDCM 00024) i sedam bakterijskih kultura (Escherichia coli, Providencia stuartii, Pseudomonas spp. Β-hemolitična Escherichia coli, koagulaza pozitivan stafilokok, Staphylococcus aureus, Sreptococcus grupe D) iz kolekcije Laboratorije za mikrobiologiju Veterinarskog instituta „Dr Vaso Butozan“ Banja Luka. Rezultati rada su potvrdili da ekstrakt bijelog ljiljana pokazuje određenu antibakterijsku aktivnost prema ispitanim patogenima korištenim u ovom istraživanju. Antibakterijska aktivnost ekstrakta bijelog ljiljana se kretala u opsegu od 5,33 mm do 18,88 mm, zavisno od bakterijskog soja i koncentracije ekstrakta bijelog ljiljana.

Natural extracts made from fresh plant material and biologically active compounds isolated from different plant species that have been used in folk medicine for centuries, may present valuable sources for production of novel natural preservatives and medicinal preparations. White lily-Lilium candidum is also used, for a long period of time, in folk medicine for healing of burns and frostbites, otitis and rhinitis as well as balm for washing wounds and cuts. Roots, leaves and flowers have medical properties. Nevertheless, there is little information in literature related to antibacterial effect of white lily extract on certain pathogens and there is a need to determine the justification for its use in traditional medicine. Antibacterial activity of White lily extract on five reference cultures (Escherichia coli WDCM 00090, Listeria monocytogenes WDCM 00020, Salmonella enterica WDCM 00030, Pseudomonas aeruginosa WDCM 00024) and seven bacterial cultures (Escherichia coli, Providencia stuartii, Pseudomonas spp. ß-hemolytic Escherichia coli, coagulase positive staphylococcus, Staphylococcus aureus, group D Sreptococcus) from Laboratory for Microbiology, Public Veterinary Institute of Republic of Srpska "Dr. Vaso Butozan", Banja Luka, were examined in this study. Results confirmed that white lily extract show certain antibacterial activity against examined pathogens used in this study. Antibacterial activity of White lily extract was in the range from 5.33 mm to 18.88 mm depending of bacterial strain and concentration of white lily extract.