Seed germination is a critical step in the life cycle of plants controlled by the phytohormones abscisic acid (ABA) and gibberellin (GA), and by phytochromes, an important class of photoreceptors in plants. Here we show that light-dependent germination is enhanced in mutants deficient in the AP2/ERF transcription factors ERF55 and ERF58. Light-activated phytochromes repress ERF55/ERF58 expression and directly bind ERF55/ERF58 to displace them from the promoter of PIF1 and SOM, genes encoding transcriptional regulators that prevent the completion of germination. The same mechanism controls the expression of genes that encode ABA or GA metabolic enzymes to decrease levels of ABA and possibly increase levels of GA. Interestingly, ERF55 and ERF58 are themselves under transcriptional control of ABA and GA, suggesting that they are part of a self-reinforcing signalling loop which controls the completion of germination. Overall, we identified a role of ERF55/ERF58 in phytochrome-mediated regulation of germination completion.

Appropriate timing of seed germination is crucial for plant survival and has important implications for agricultural production. Timely germination relies on harmonious interactions between endogenous developmental signals, especially abscisic acid (ABA) and gibberellins (GAs), and environmental cues such as light. Recently, a series of investigations with regard to the crosstalk between phytochromes, ABA, and GAs in the regulation of seed germination demonstrated that the transcription factor ABSCISIC ACID INSENSITIVE 5 (ABI5) is a central mediator in the light–ABA/GA cascades. Here, we review the information on ABI5 as a key player in light-, ABA-, and GA-signaling pathways to precisely control seed germination. We highlight the recent advances in ABI5-related studies, focusing on the regulation of seed germination, which is strictly controlled at both transcriptional and protein levels by numerous light-regulated factors. We further discuss the components of ABA- and GA-signaling pathways that could regulate ABI5 during seed germination, including transcription factors, E3 ligases, protein kinases, and phosphatases. The precise molecular mechanisms by which ABI5 mediates ABA–GA antagonistic crosstalk during seed germination are also described. Finally, some potential research hotspots underlying ABI5-mediated seed germination regulatory networks are proposed.

Coding metasurfaces have attracted tremendous interests due to unique capabilities of manipulating electromagnetic wave. However, archiving transmissive coding metasurface is still challenging. Here we propose a transmissive anisotropic coding metasurface that enables the independent control of two orthogonal polarizations. The polarization beam splitter and the OAM generator have been studied as typical applications of anisotropic 2-bit coding metasurface. The simulated far field patterns illustrate that the x and y polarized electromagnetic waves are deflected into two different directions, respectively. The anisotropic coding metasurface has been experimentally verified to realize an orbital angular momentum (OAM) beam with l = 2 of right-handed polarized wave, resulting from both contributions from linear-to-circular polarization conversion and the phase profile modulation. This work is beneficial to enrich the polarization manipulation field and develop transmissive coding metasurfaces.

Summary An environmentally responsive root system is crucial for plant growth and crop yield, especially in suboptimal soil conditions. This responsiveness enables the plant to exploit regions of high nutrient density while simultaneously minimizing abiotic stress. Despite the vital importance of root systems in regulating plant growth, significant gaps of knowledge exist in the mechanisms that regulate their architecture. Auxin defines both the frequency of lateral root (LR) initiation and the rate of LR outgrowth. Here, we describe a search for proteins that regulate root system architecture (RSA) by interacting directly with a key auxin transporter, PIN1. The native separation of Arabidopsis plasma membrane protein complexes identified several PIN1 co‐purifying proteins. Among them, AZG1 was subsequently confirmed as a PIN1 interactor. Here, we show that, in Arabidopsis, AZG1 is a cytokinin (CK) import protein that co‐localizes with and stabilizes PIN1, linking auxin and CK transport streams. AZG1 expression in LR primordia is sensitive to NaCl, and the frequency of LRs is AZG1‐dependent under salt stress. This report therefore identifies a potential point for auxin:cytokinin crosstalk, which shapes RSA in response to NaCl.