INTRODUCTION: At present, the common telemedicine service quality evaluation methods can not obtain the key evaluation indicators, which leads to the low accuracy and low user satisfaction. OBJECTIVES: This paper constructs a telemedicine service quality evaluation model based on machine vision technology. METHODS: Machine vision technology is used to obtain telemedicine service information, preliminarily select service quality assessment indicators, complete the selection of indicators, build a telemedicine service quality assessment indicator system, adopt subjective and objective combination method to calculate the weight of service quality assessment indicators, and combine matter element analysis method to build a telemedicine service quality assessment model. RESULTS: The experimental results show that the Cronhach a is higher than 0.7, the Barthel index is higher than 90, and the satisfaction of many users is more than 90%. CONCLUSION: The proposed method solves the problems existing in the current method and lays a foundation for the development of telemedicine service technology.