Aims:The objective of this research is to study the preventive effectiveness of Nigella sativa L. against rat kidney stones, generated by Ethylene Glycol. Methodology: In this study, 40 Wistar rats were categorized randomly into four groups of ten. During the research, drinking water labeled group A is mixed with mineral water through utilization Original Research Articleof positive control procedures. Into the drinking water labeled group B and other research groups, 1 percent Ethylene Glycol is added through the administration of negative control. In preventive group C, Nigella sativa is added from the first day of the research period and in the treatment of group D. After the 15 th day of the research period, 750 mg/kg Nigella sativa powder is administered into the drinking water of rats per day. At the end of the research, kidney tissue samples of rats were stained with haematoxylin and Eosin through the utilization of an optical microscope. Furthermore, serum and urinary samples of rats were analyzed biochemically. Results:The results indicated that the number of Calcium Oxalate crystals in group B increased in comparison with that of group A. Biochemical analysis of serum and urinary samples indicated a significant increase in the number of Calcium Oxalate crystals of group B in comparison with group A. Furthermore, the analyses depict a unanimous decrease of crystals in all the research groups (except in group C) in comparison with group B. Conclusion:The findings of this research indicate that Nigella sativa L. does not have any preventive effectiveness against Calcium Oxalate accumulation.

The treatment of alkylated nitro derivatives of indazole with 2-(4-chlorophenyl)acetonitrile under basic conditions gave the new 8-chloro-3-alkyl-3H-pyrazolo[4,3-a]acridine-11-carbonitriles via the nucleophilic substitution of hydrogen which proceeds at room temperature with concomitant cyclisation in fairly good yields. The structures of all newly synthesized compounds were confirmed by IR, 1 H NMR, 13 C NMR and mass spectral data. Fluorescence experimental results of all newly synthesized compounds revealed remarkable photoluminescence properties and strong green fluorescence properties. Also, the new compounds exhibited potent antibacterial activity and their antibacterial activity (MIC) against Gram positive (Staphylococcuse aureus methicillin resistant S. aureus and Bacillus subtilis) and negative bacterial (Pseudomonas aeruginosa and Escherichia coli) species were determined.