The aim of this study was to determine the pathogenic agents, which have been isolated in ICUs and determine their antimicrobial susceptibility in the first two years of our hospital. Methods: A total 368 strains (194 Pseudomonas aeruginosa, and 174 Acinetobacter baumannii) that were considered as an infectious agent and isolated from different clinical samples of hospitalized patients in intensive care units between January 2011 and December 2012 were included in the study. Conventional methods and automatized system were used for the identification and antibiotic susceptibility of the isolates with according to guidelines of Clinical and Laboratory Standarts Institute (CLSI). Imipenem and meropenem resistance has been confirmed by the E test method and interpreted according to CLSI. Results: Resistance rates of P.aeruginosa strains were as follows: Meropenem 35%, imipenem 36%, cefepim 21%, ceftazidim and ciprofloxacin 29%, gentamicin 33%, piperacilin/tazobactam 35%, amikacin 37%. Resistance rates of A.baumannii strains were as follows: Meropenem 85%, imipenem 87%, amikacin 22%, trimethoprim/sulfamethoxazole 25%, gentamicin 46%, piperacilin/tazobactam 54%, ampicilin/sulbactam 59%, cefepim 64%, ciprofloxacin 71%, ceftazidim 83%. Conclusion: Increased carbapenem resistance in our ICUs demonstrates importance of antimicrobial susceptibility tests. The antibiotic susceptibility tests should be surveyed continuously to avoid the spread of intensive care unit isolates carrying high level antibiotic resistance for better achievement of treatment regimens.

ÖzGiriş: Bu çalışmada otoimmün hastalıkların tanısı için laboratuvarımıza gelen örnekler ile yapılan İndirekt Floresan Antikor (IFA) sonuçları değerlendirilmiştir. Gereçler ve AbstractIntroduction: The present study evaluated the results of indirect fluorescent antibody (IFA) method performed in the specimens sent to our laboratory to be diagnosed in terms of autoimmune diseases.