It has been reported that electromagnetic fields (EMFs) can promote the healing of non-union, osteogenesis and differentiation of the osteoblasts. However, its mechanism has not been unravelled. In this study, we detected some response induced by EMF and evaluated the importance of these signals for EMF-induced osteogenesis in bone marrow mesenchymal stem cells (MSCs). We characterized the expression of EMF-induced osteogenesis markers in MSCs, using RT-PCR and real-time PCR. Western blot was used to detect the signalling pathways. We found that EMF could promote osteogenesis in MSCs, along with the expression of several osteogenic markers. EMF-induced cyclic adenosine monophosphate (cAMP) level increase causes protein kinase A (PKA) and extracellular signal-regulated kinase (ERK)1/2 phosphorylation. Pretreating the MSCs with the mitogen-activated protein kinase (MAPK)/ERK kinase 1/2 (MEK1/2) inhibitor PD98059, or the PKA inhibitor H-89, significantly inhibited the induction of osteogenic markers, showing that EMF induction of osteogenesis was dependent on the ERK and PKA signalling pathways. Therefore, our study showed that EMF promoted MSC osteogenesis and that the EMF-induced osteogenic markers were mediated by both the PKA and MAPK signalling pathways. Copyright © 2014 John Wiley & Sons, Ltd.

The aim of this retrospective study is to review our experience in the diagnosis and role of thoracotomy for traumatic diaphragmatic hernia (TDH). Between January 2008 and June 2014, 23 patients from Yangzhou Medical College (Yangzhou China) and Lishui Center Hospital (Lishui China), who underwent thoracotomy for TDH, were analyzed. The clinical features, imaging findings, operative findings, and outcome of treatment in these patients are presented. There were 23 patients (18 males and 5 females) who underwent surgical procedures due to TDH. The median age of the patients was 43.2 years (range, 15-68 years). The cause of rupture was penetrating trauma in 1 (4.3 %) patient and blunt trauma in 22 (95.7 %) patients. The TDH was left sided in 21 patients and right sided in two patients. The diagnosis was made by chest X-ray (n = 2) and chest or abdominal CT (n = 13) and at thoracotomy based on a high index of suspicion (n = 8). Associated injuries were seen in 21 patients (91.3 %). Twenty-two patients underwent thoracotomy, and one underwent thoracotomy with laparotomy. The mean operating time was 112 min (range, 60-185 min) and the mean blood loss was 116 mL (range, 20-400 mL). The most common herniated organs were the omentum (n = 15), stomach (n = 14), spleen (n = 11), colon (n = 10), small bowel (n = 2), and liver (n = 1). All diaphragmatic defects were repaired using interrupted prolene sutures. The overall mortality rate was 4.3 % (n = 1). The diagnosis of TDH is easily missed or delayed. Chest X-ray and computer tomography (CT), especially chest and abdominal CT, are useful in the diagnosis of diaphragmatic ruptures, and thoracotomy is an effective and successful treatment for TDH.

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