Metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) is a type of long noncoding RNA. It is associated with metastasis and is a favorable prognostic factor for lung cancer. Recent studies have shown that MALAT1 plays an important role in other malignancies. But, little is known about the role of MALAT1 in glioma. In this study, quantitative reverse transcription PCR (qRT-PCR) was used to demonstrate that the expression of MALAT1 was lower than that in normal brain tissues. Stable RNA interference-mediated knockdown of MALAT1 in human glioma cell lines (U87 and U251) significantly promoted the invasion and proliferation of the glioma cells by in vitro assays. Conversely, overexpression of MALAT1 caused significant reduction in cell proliferation and invasion in vitro, and tumorigenicity in both subcutaneous and intracranial human glioma xenograft models. Furthermore, MALAT1-mediated tumor suppression in glioma cells may be via reduction of extracellular signal-regulated kinase/mitogen-activated protein kinase (ERK/MAPK) signaling activity and expression of matrix metalloproteinase 2 (MMP2). In conclusion, overall data demonstrated the tumor-suppressive role of MALAT1 in glioma by attenuating ERK/MAPK-mediated growth and MMP2-mediated invasiveness.

A multi-dimensional Eulerian two-phase model for sediment transport, called SedFoam, is presented. The model was developed under the open-source framework via the CFD toolbox OpenFOAM. With closures of particle stresses and fluid-particle interactions, the model is able to resolve processes in the concentrated region of sediment transport and hence does not require conventional bedload/suspended load assumptions. A modified k − closure was adopted for the carrier flow turbulence. The model was validated for Reynolds-averaged steady and oscillatory sheet flows and verified with empirical formulae for scour downstream of an apron. The model was used to study momentary bed failure (or plug flow) under sheet flow conditions. Model results revealed the existence of instabilities of the near-bed transport layer when momentary bed failure criteria was exceeded. These instabilities evolved into 5 − 10 cm billows and were responsible for the large transport rate. The instabilities were associated with a large erosion depth, which was triggered by the combination of large bed shear stresses and large horizontal pressure gradients. Further numerical experiments confirmed the conjecture by previous studies that a criterion for onset of momentary bed failure in oscillatory sheet flow was a function of both the Shields parameter and Sleath parameter.

Abstract. In this paper, a three-dimensional two-phase flow solver, SedFoam-2.0, is presented for sediment transport applications. The solver is extended from twoPhaseEulerFoam available in the 2.1.0 release of the open-source CFD (computational fluid dynamics) toolbox OpenFOAM. In this approach the sediment phase is modeled as a continuum, and constitutive laws have to be prescribed for the sediment stresses. In the proposed solver, two different intergranular stress models are implemented: the kinetic theory of granular flows and the dense granular flow rheology µ(I ). For the fluid stress, laminar or turbulent flow regimes can be simulated and three different turbulence models are available for sediment transport: a simple mixing length model (one-dimensional configuration only), a k − ε, and a k − ω model. The numerical implementation is demonstrated on four test cases: sedimentation of suspended particles, laminar bed load, sheet flow, and scour at an apron. These test cases illustrate the capabilities of SedFoam-2.0 to deal with complex turbulent sediment transport problems with different combinations of intergranular stress and turbulence models.

Previous field observations revealed that the wave boundary layer is one of the main conduits delivering fine sediments from the nearshore to continental shelves. Recently, a series of turbulenceresolving simulations further demonstrated the existence of a range of flow regimes due to different degrees of sediment-induced density stratification controlled by the sediment availability. In this study, we investigate the scenario in which sediment availability is governed by the resuspension/deposition from/to the bed. Specifically, we focus on how the critical shear stress of erosion and the settling velocity can determine the modes of transport. Simulations reveal that at a given wave intensity, which is associated with more energetic muddy shelves and a settling velocity of about 0.5 mm/s, three transport modes, ranging from the well-mixed transport (mode I), two-layer like transport with the formation of lutocline (mode II), and laminarized transport (mode III) are obtained as the critical shear stress of erosion reduces. Moreover, reductions in the settling velocity also yield similar transitions of transport modes. We also demonstrate that the onset of laminarization can be well explained by the reduction of wave-averaged bottom stress to about 0.39 Pa due to attenuated turbulence by sediments. A 2-D parametric map is proposed to characterize the transition from one transport mode to another as a function of the critical shear stress and the settling velocity at a fixed wave intensity.