Separating point clouds into ground and nonground points is an essential step in the processing of airborne laser scanning (ALS) data for various applications. Interpolation-based filtering algorithms have been commonly used for filtering ALS point cloud data. However, most conventional interpolation-based algorithms have exhibited a drawback in terms of retaining abrupt terrain characteristics, resulting in poor algorithmic precision in these regions. To overcome this drawback, this paper proposes an improved adaptive surface interpolation filter with a multilevel hierarchy by using a cloth simulation and relief amplitude. This method uses three hierarchy levels of provisional digital elevation model (DEM) raster surfaces with thin plate spline (TPS) interpolation to separate ground points from unclassified points based on adaptive residual thresholds. A cloth simulation algorithm is adopted to generate sufficient effective initial ground seeds for constructing topographic surfaces with high quality. Residual thresholds are adaptively constructed by the relief amplitude of the examined area to capture complex landscape characteristics during the classification process. Fifteen samples from the International Society for Photogrammetry and Remote Sensing (ISPRS) commission are used to assess the performance of the proposed algorithm. The experimental results indicate that the proposed method can produce satisfying results in both flat areas and steep areas. In a comparison with other approaches, this method demonstrates its superior performance in terms of filtering results with the lowest omission error rate; in particular, the proposed approach retains discontinuous terrain features with steep slopes and terraces.

In this paper, we present a refinement method for cryo-electron microscopy (cryo-EM) single-particle reconstruction, termed as OPUS-SSRI (Sparseness and Smoothness Regularized Imaging). In OPUS-SSRI, spatially varying sparseness and smoothness priors are incorporated to improve the regularity of electron density map, and a type of real space penalty function is designed. Moreover, we define the back-projection step as a local kernel regression and propose a first-order method to solve the resulting optimization problem. On the seven cryo-EM datasets that we tested, the average improvement in resolution by OPUS-SSRI over that from RELION 3.0, the commonly used image-processing software for single-particle cryo-EM, was 0.64 Å, with the largest improvement being 1.25 Å. We expect OPUS-SSRI to be an invaluable tool to the broad field of cryo-EM single-particle analysis. The implementation of OPUS-SSRI can be found at https://github.com/alncat/cryoem.

Exploring the spatial patterns of COVID-19 transmission and its key determinants could provide a deeper understanding of the evolution of the COVID-19 pandemic. The goal of this study is to investigate the spatial patterns of COVID-19 transmission in different periods in Singapore, as well as their relationship with demographic and built-environment factors. Based on reported cases from 23 January to 30 September 2020, we divided the research time into six phases and used spatial autocorrelation analysis, the ordinary least squares (OLS) model, the multiscale geographically weighted regression (MGWR) model, and dominance analysis to explore the spatial patterns and influencing factors in each phase. The results showed that the spatial patterns of COVID-19 cases differed across time, and imported cases presented a random pattern, whereas local cases presented a clustered pattern. Among the selected variables, the supermarket density, elderly population density, hotel density, business land proportion, and park density may be particular fitting indicators explaining the different phases of pandemic development in Singapore. Furthermore, the associations between determinants and COVID-19 transmission changed dynamically over time. This study provides policymakers with valuable information for developing targeted interventions for certain areas and periods.

Traffic congestion, especially during peak hours, has become a challenge for transportation systems in many metropolitan areas, and such congestion causes delays and negative effects for passengers. Many studies have examined the prediction of congestion; however, these studies focus mainly on road traffic, and subway transit, which is the main form of transportation in densely populated cities, such as Tokyo, Paris, and Beijing and Shenzhen in China, has seldom been examined. This study takes Shenzhen as a case study for predicting congestion in a subway system during peak hours and proposes a hybrid method that combines a static traffic assignment model with an agent-based dynamic traffic simulation model to estimate recurrent congestion in this subway system. The homes and work places of the residents in this city are collected and taken to represent the traffic demand for the subway system of Shenzhen. An origin-destination (OD) matrix derived from the data is used as an input in this method of predicting traffic, and the traffic congestion is presented in simulations. To evaluate the predictions, data on the congestion condition of subway segments that are released daily by the Shenzhen metro operation microblog are used as a reference, and a comparative analysis indicates the appropriateness of the proposed method. This study could be taken as an example for similar studies that model subway traffic in other cities.

Many Cryo-EM datasets contain structural heterogeneity due to functional or nonfunctional dynamics that conventional reconstruction methods may fail to resolve. Here we propose a new method, OPUS-DSD (Deep Structural Disentanglement), which can reliably reconstruct the structural landscape of cryo-EM data by directly translating the 2D cryo-EM images into 3D structures. The method adopts a convolutional neural network and is regularized by a latent space prior that encourages the encoding of structural information. The performance of OPUS-DSD was systematically compared to a previously reported method, cryoDRGN, on synthetic and real cryo-EM data. It consistently outperformed existing methods, resolved large or small structural heterogeneity, and improved the final reconstructions of tested systems even on highly noisy cryo-EM data. The results have shown that OPUS-DSD should be particularly suitable for cases in which the high structural flexibilities cannot easily be represented by rigid-body movements. Therefore, OPUS-DSD represents a valuable tool that can not only recover functionally-important structural dynamics missed in a traditional cryo-EM refinement, but also improve the final reconstruction by increasing homogeneity in a dataset.