A novel synthetic copolymer (PAA) for bone repair was prepared by melt condensation polymerization with 6-aminohexanoic acid (He) and 4R-hydroxy-L-proline (Hyp). The structure and thermal property were characterized by infrared spectrometer (IR), nuclear magnetic resonance (H1NMR) and differential scanning calorimeter (DSC). The results indicated that the PAA had amide linkages in their polymer chains. The Tg of PAA was 57.56°C and significantly higher than that of nylon6, 50.46°C. Meanwhile, the intrinsic viscosity and mechanical properties were investigated at different He/Hyp ratios. It revealed that appropriate introduction of Hyp group could control the degree of polycondensation and adjusted mechanical properties of PAA obviously close to natural bone. The prepared polymers had about 2.12~18.21 % weight loss after 8-week soaking in PBS, showed degradable properties which was essentials to new bone growth. The copolymer of He and Hyp with ratios of 90/10 had 6.57 % weight loss and maintained the yield compressive strengths with about 67.51 MPa after 8 weeks, which exhibited a compatible mechanical properties and degradation speed for bone repair.
Lespedeza bicolor has developed root system and the ability of carbon sequestration, which can effectively improve the soil nutrient. We measured the correlation of net photosynthetic rate and physiological and environmental factors using space mutated Lespedeza bicolor of SP1 generation, the change of soil carbon and nitrogen content and microbial biomass was also discussed. The conclusion showed that the diurnal variation of photosynthesis was registered as double-peak curve and had "noon break" phenomenon. It showed strong ability of photosynthesis and its mean diurnal photosynthetic rates was 8.77 umolCO2·m-2s-1. Highly correlation was existed between net photosynthetic rate and atmospheric CO2 concentration ,the content of soil organic carbon and total nitrogen and soil microbial biomass displayed negatively relationship to the depth of the soil. Base on the study , we can conclude that the photosynthetic organic matter was effectively accumulated by Lespedeza bicolor of SP1 generation which made use of atmospheric CO2 and the root system located in the superficial soil has better potential of carbon sequestration.
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