Accumulating data from genome-wide association studies (GWAS) have provided a collection of novel candidate genes associated with complex diseases, such as atherosclerosis. We identified an atherosclerosis-associated single-nucleotide polymorphism (SNP) located in the intron of the long noncoding RNA (lncRNA) LINC00305 by searching the GWAS database. Although the function of LINC00305 is unknown, we found that LINC00305 expression is enriched in atherosclerotic plaques and monocytes. Overexpression of LINC00305 promoted the expression of inflammation-associated genes in THP-1 cells and reduced the expression of contractile markers in co-cultured human aortic smooth muscle cells (HASMCs). We showed that overexpression of LINC00305 activated nuclear factor-kappa beta (NF-κB) and that inhibition of NF-κB abolished LINC00305-mediated activation of cytokine expression. Mechanistically, LINC00305 interacted with lipocalin-1 interacting membrane receptor (LIMR), enhanced the interaction of LIMR and aryl-hydrocarbon receptor repressor (AHRR), and promoted protein expression as well as nuclear localization of AHRR. Moreover, LINC00305 activated NF-κB exclusively in the presence of LIMR and AHRR. In light of these findings, we propose that LINC00305 promotes monocyte inflammation by facilitating LIMR and AHRR cooperation and the AHRR activation, which eventually activates NF-κB, thereby inducing HASMC phenotype switching.

The traditional nonabsorbable silicone buckling implant (buckle) may cause some long-term complications when it is used in the retinal detachment surgery. In this study we fabricated a chitosan-gelatin based buckling implant to replace the traditional one. We evaluated its biocompatibility with human scleral fibroblasts (HSF) in vitro and its cytotoxicity with L929 cell. To evaluate elasticity and hardness of the blends buckle, the mechanical properties of the chitosan-gelatin buckle were compared with the traditional silicone buckle. The light and electron microscopy coupled with immunocytochemistry demonstrated that chitosan-gelatin blends supported the survival and growth of primarily cultured HSF without significant cytotoxic effects. MTT analysis and cell cycle analysis indicated that chitosan-gelatin blends promoted the proliferation of HSF. A preliminary in vivo implantation test indicated that chitosan-gelatin buckling implant were compatible with the surrounding tissue. The results collectively demonstrated that chitosan-gelatin blends could be a candidate biodegradable material for scleral buckling surgery.

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Regional economic vitality reflects the scale and development potential of a region’s economy. It largely determines the development of the city, and is also affected by many factors such as population competitiveness, corporate competitiveness, market vitality, innovation vitality, and environmental vitality. A pilot model was constructed with Hebei Province as the inspection area. Quantitative measurement of regional economic vitality was made by finding 21 indicators that indirectly or indirectly affect the economic vitality of Hebei Province. By analyzing the data of 21 indicators for nearly 10 years, the time series clustering is used to achieve the dimensionality reduction of the indicators. After the dimension reduction, it is divided into four categories: overall scale, development potential, market vitality, and innovation vitality. Construct the economic vitality structure model of Hebei Province, and determine the four types of contribution to economic vitality and compare them. On this basis, more accurately grasp the indicators that affect economic vitality and work out reasonable and effective action plans. From the perspective of human resources and corporate vitality, analyze how the action plan accurately affects the economic vitality of Hebei Province[1]. The 11 cities in Hebei Province are the target of regional economic vitality. The economic vitality structure model constructed uses the required contribution value to select priority indicators. Finally, the six indicators of GPD, GPD growth rate, fiscal revenue, fiscal revenue growth rate, number of industrial enterprises above designated size, and total profit of industrial enterprises above designated size were established for eleven cities in Hebei Province to construct a TOPSIS scoring model, and calculation rankings were conducted through MATLAB. Results The top three cities were Shijiazhuang, Tangshan and Cangzhou.