Fabrication and evaluation of chitosan–gelatin based buckling implant for retinal detachment surgery

Abstract

The traditional nonabsorbable silicone buckling implant (buckle) may cause some long-term complications when it is used in the retinal detachment surgery. In this study we fabricated a chitosan-gelatin based buckling implant to replace the traditional one. We evaluated its biocompatibility with human scleral fibroblasts (HSF) in vitro and its cytotoxicity with L929 cell. To evaluate elasticity and hardness of the blends buckle, the mechanical properties of the chitosan-gelatin buckle were compared with the traditional silicone buckle. The light and electron microscopy coupled with immunocytochemistry demonstrated that chitosan-gelatin blends supported the survival and growth of primarily cultured HSF without significant cytotoxic effects. MTT analysis and cell cycle analysis indicated that chitosan-gelatin blends promoted the proliferation of HSF. A preliminary in vivo implantation test indicated that chitosan-gelatin buckling implant were compatible with the surrounding tissue. The results collectively demonstrated that chitosan-gelatin blends could be a candidate biodegradable material for scleral buckling surgery.

Efficacy, Safety, and Biodegradation of a Degradable Scleral Buckle of Chitosan–gelatin Polymer in Rabbits

“…32 And such CS-Gel complex was proved having suitable mechanical properties compared with silicone sponge. 11 Although there is still no consensus on the optimal duration of indentation needed to ensure surgical effect, 6 months is recommended as the minimum time for determining functional success of operative treatment of RD. 33 Rapid biodegradation of the scleral buckles before the neural retina attaching to the underlying retinal pigment epithelium solidly may cause the failure of the surgical treatment.…”

“…11 CS-Gel were prepared by dissolving CS (Nantong Xincheng Biochemical Company, Jiangsu, China) in 2% (vol/vol) acetic acid and then adding dissolved Gel to the CS solution in a weight ratio of 25% CS/75% Gel. The well-mixed paste was packed into a cylindrical glass tube (8 mm in diameter, 100 mm in length) using a syringe.…”

“…11 The goal of the current work was to use rabbit as a model to characterize CS-Gel in terms of efficacy, safety, and biodegradation as a scleral buckle in comparison with clinically used silicone sponge.…”

Preparation, Properties and Applications of Chitosan-Based Biocomposites/Blend Materials: A Review

“…This approach seems to be more useful in sustaining a wound healing especially the one that needs longer recovery time. Meanwhile, several works have been directed towards preparing Cs blended poly-L-lysine [356], polyethylene glycol [357] or essential oil [358] films and found that this combination has significantly improved nerve cell affinity compared to native Cs as indicated by the increase in attachment, differentiation and growth of nerve cells. Through this invention, it can be claimed that the produced film is an even better material in nerve cell affinity as compared to Cs/collagen composites.…”

Investigation of osteoblast cells behavior in polymeric 3D micropatterned scaffolds using digital holographic microscopy

“…In recent years, the behavior of cells in contact with polymeric scaffolds has been the subject of numerous studies, especially due to its impact for applications such as tissue engineering, implants, and drug delivery [1][2][3]. Other multidisciplinary fields, which combine microtechnologies and life sciences, have also developed an interest in such studies, aiming to improve the quality of the scaffolds, with the final goal to achieve conditions similar to the in vivo environment.…”