As a powerful self-assembly strategy, DNA origami technique has found numerous applications in various areas. Many of the applications require pure and dense DNA origami solutions, therefore, the post-assembly treatment of purification and concentration is essentially required. Herein, we reported a fast and facile approach for simultaneously purifying and concentrating DNA origami objects through ethanol precipitation. The triangular and rectangular DNA origami nanostructures have been successfully purified and concentrated using our approach. Our work provides a simple yet efficient way for preparing concentrated and purified DNA origami, which is expected to be applicable to the scalable production of pure and dense DNA origami solutions.

Cations such as divalent magnesium ion (Mg 2 + ) play an essential role in DNA self-assembly. However, the strong electrostatic shielding effect of Mg 2 + would be disadvantageous in some situations that require relatively weak interactions to allow a highly reversible error-correcting mechanism in the process of assembly. Herein, by substituting the conventional divalent Mg 2 + with monovalent sodium ion (Na + ), we have achieved one-pot high-yield assembly of tile-based DNA polyhedra at micromolar concentration of tiles, at least 10 times higher than the DNA concentrations reported previously. This strategy takes advantage of coexisting counterions and is expected to surmount the major obstacle to potential applications of such DNA nanostructures: large-scale production.

Controlling the self‐assembly of DNA nanostructures using rationally designed logic gates is a major goal of dynamic DNA nanotechnology, which could facilitate the development of biomedicine, molecular computation, et al. In previous works, the regulations mostly relied on either toehold‐mediated strand displacement or stimuli‐driven conformational switch, requiring elaborately‐designed or specific DNA sequences. Herein, we reported a facile, base‐sequence‐independent strategy for logically controlling DNA self‐assembly through external molecules. The INHIBIT and XOR logic controls over the assembly/disassembly of DNA polyhedra were realized through cystamine (Cyst) and ethylenediamine (EN) respectively, which were further integrated into a half subtractor circuit thanks to the sharing of the same inputs. Our work provides a sequence‐independent strategy in logically controlling DNA self‐assembly, which may open up new possibilities for dynamic DNA nanotechnology.