Background: Long noncoding RNAs (LncRNA) are emerging as key regulators in various biological processes. However, their role in epithelial-to-mesenchymal transition (EMT) remains elusive. Results: A subset of lncRNAs are dysregulated upon transforming growth factor (TGF) ␤-induced EMT, and lncRNA-HIT mediates this process. Conclusion: LncRNAs such as lncRNA-HIT ((HOXA transcript induced by TGF␤) play a pivotal role in EMT and breast cancer progression. Significance: Here we profiled lncRNAs in TGF␤-induced EMT and identified a novel conserved lncRNA-HIT.