In this article we proposed a simple hexagonal model for exploring the hybridization of thiol-modified probe DNA self-assembled monolayers (SAMs) on gold with target DNA molecules in solution. The size-fitting coefficient d(c)/d(t) from the model was used to discuss the principle for DNA optimal hybridization, where d(c) was the channel diameter among three adjacent probe DNA molecules on gold and dt was the gyration diameter of the target DNA molecules in solution. Experimentally we investigated the hybridization effect (hybridization efficiency H(E) and hybridization density H(D)) of thiol-modified probe DNA (DNA base amount m = 15, 25 or 35)/mercaptohexanol (MCH) mixed SAMs on gold in 1 M electrolyte solution by chronocoulometry (CC) and electrochemical impedance spectroscopy (EIS). The surface coverage Γ(m) of the probe DNA on gold was adjusted by changing the mixed concentration ratio of probe DNA with MCH (C(DNA)/C(MCH)) in the assembly solution. Results indicated that with the increase of C(DNA)/C(MCH), H(E) decreased gradually; H(D) first increased and then decreased, which arrived at the biggest at C(DNA)/C(MCH) = 1 for all the probe DNA/MCH mixed SAMs (m = 15, 25, 35). The optimal Γ(m) for achieving the biggest H(D) in DNA hybridization decreased with the increase of m from 15 to 35. The experimental conclusions obtained by CC and EIS measurements verified each other. Combining the simple model with our experimental results, we ascertained that d(c)/d(t) decreased with the increase of m, which showed a good linear relationship. These conclusions provided an important reference and guidance for controllably constructing DNA sensors with optimal performance.

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Forest structural parameters are key indicators for forest growth assessment, and play a critical role in forest resources monitoring and ecosystem management. Terrestrial laser scanning (TLS) can obtain three-dimensional (3D) forest structures with ultra-high precision without destruction, whereas some shortcomings such as non-portability and cost-consuming can limit the quick and broad acquisition of forest structure. Structure from motion (SfM) and backpack laser scanning (BLS) technology have the advantages of low-cost and high-portability while obtaining 3D structure information of forests. In this study, the high-overlapped images and the BLS point cloud, combined with the point cloud registration and individual tree segmentation to extract the forest structural parameters and compared with the TLS for assessing the accuracy and efficiency of low-cost SfM and portable BLS point clouds. Three plots with different forest structural complexity (coniferous, broadleaf and mixed plot) in the northern subtropical forests were selected. Firstly, portable photography camera, BLS and TLS were used to acquire 3D SfM and LiDAR point clouds, and spatial co-registration of different-sourced point cloud datasets were carried out based on the understory markers. Secondly, the point clouds of individual tree trunk and crown were segmented by the comparative shortest-path algorithm (CSP), and then the height and position of individual tree were extracted based on the tree crown point cloud. Thirdly, the trunk diameter at different heights were calculated by point cloud slices using the density-based spatial clustering of applications with noise (DBSCAN) algorithm, and combined with the stem curve of individual tree which was constructed using four Taper equations to estimate the individual tree volume. Finally, the extraction accuracy of forest structural parameters based on SfM and BLS point clouds were verified and comprehensively compared with field-measured and TLS data. The results showed that: (1) the individual tree segmentation based on SfM and BLS point clouds all performed quite well, among which the segmentation accuracy (F) of SfM point cloud was 0.80 and the BLS point cloud was 0.85; and (2) the accuracy of DBH and tree height extraction based on the SfM and BLS point clouds in comparison with the field-measured data were relatively high. The root mean square error (RMSE) of DBH and tree height extraction based on SfM point cloud were 2.15 cm and 4.08 m, and the RMSE of DBH and tree height extraction based on BLS point cloud were 2.06 cm and 1.63 m. This study shows that with the adopted image capture method, terrestrial SfM photogrammetry can be applied quite well in extracting DBH.