Background: Various medicinal plants have much efficacious value as treatment of many fatal conditions including cancer. Kigelia africana has known therapeutic efficacy in different ailments and has been used as traditional medicine since ages. Aim: To evaluate anticancer property of any drug or plant extract including HepG2 cell line. Methodology: Anticancer activity of n-hexane and ethanolic extracts of Kigelia africana was checked. IC50 was evaluated via MTT assay, crystal violet assay was performed to check the viability of cells and trypan blue assay to count dead cells. Furthermore, muse analysis was performed using count and viability kit to count total living as well non-living cells. Results: Cancer cells of HepG2 cell line showed reduced viability and proliferation with increased apoptosis when treated with Kigelia extracts. Conclusion: Many drugs have been proved as anticancer but with severe side effects. Thus, phytoextracts have been tested in this study to evaluate their anti-cancer activity so minimize the side effect. Kigelia africana extracts were found to be effective against liver cancer cells. Keyword: Kigelia africana, HepG2, MTT, liver cancer, anticancer drug, plant based drugs.

Background: Infection of Hepatitis C virus (HCV) is major contributor in liver problems, affecting nearly more than 70 million people all over the world. Top ten countries which are affected by Hepatitis, also include Pakistan. Medical science has entered a new era of HCV therapy in elimination of infection and disease is a real possibility. In Asia, Genotype 3 is chronic hepatitis C genotype as compared to other genotypes. Direct-acting antiviral drugs are famous for their high efficacy rate, but less work has done in Asia. Methods: A follow up research was performed to evaluate 3 months treatment outcome of some direct acting antiviral drugs in 35 patients (26 patients of HCV genotype 3) below 30 years of age using some relevant biological parameters. Results: A total of 30 patients yielded a pooled SVR of more than 85% after twelve weeks outcome of commonly used antiviral drugs evaluation. Conclusion: Commonly used antiviral drugs in Sialkot, Pakistan showed effectiveness in HCV patients having genotypes 1, 2, 3 and 5. Keywords: Hepatitis C virus, genotype 3, direct acting antiviral drugs, liver function tests, renal function tests

Background: Infection of Hepatitis C virus (HCV) is major contributor in liver problems, affecting nearly more than 70 million people all over the world. Top ten countries which are affected by Hepatitis, also include Pakistan. Medical science has entered a new era of HCV therapy in elimination of infection and disease is a real possibility. In asia, Genotype 3 is chronic hepatitis C genotype as compared to other genotypes. Direct-acting antiviral drugs are famous for their high efficacy rate, but less work has done in Asia. Methods: A follow up research was performed to evaluate 3 months treatment outcome of some direct acting antiviral drugs in 35 patients (26 patients of HCV genotype 3) below 30 years of age using some relevant biological parameters. Results: A total of 30 patients yielded a pooled SVR of more than 85% after twelve weeks outcome of commonly used antiviral drugs evaluation. Conclusion: Commonly used antiviral drugs in Sialkot, Pakistan showed effectiveness in HCV pts having genotypes 1, 2, 3 & 5. Keywords: Hepatitis C virus, genotype 3, direct acting antiviral drugs, liver function tests, renal function tests