This study aims to determine the polymorphism of Prolactin (PRL | XbaI and PRL | DraI) genes in Bayang ducks using the PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism) technique and its association with weight of ducks aged 1-10 weeks. This study used 200 Bayang duck blood samples consisting of 102 male ducks (♂) and 98 female ducks (♀). DNA from blood samples was isolated using the Genomic DNA Purifification Kit (Promega) using protocol from the manucfacture. The DNA was then amplified using two primers with F: 5′-AAA TTC CCT CTC ACA GTT ACA-3′ and R: 5′-GAT GCA GAG ACA AGT TTC ACC-3′ and F: 5′-GAATAGAACACTTGACCCTG-3′ and R: 5′-TAGAGGAGGCAAGCATAG-3′ which produces fragments with a length of 416 bp and 566 bp. Restriction with XbaI enzymes that recognized the binding site (5-TT CTAGA-3′) resulted 3 genotypes: homozygote (+/+), heterozygote (+/−) and homozygote (−/−) with frequencies 0.455, 0.495 and 0.050 respectively and with frequency allel (+) 0.702 and frequency allel (−) 0.297. While the results of the restriction with enzyme DraI found three types of genotypes, namely (+/+), (+/−) and (−/−) with frequencies of 0.64, 0.35 and 0.01 respectively with frequency allel (+) 0.82 and allele frequencies (−) 0.18. From the results of the analysis, it was found that there was no relationship between these two diversity and weight ducks of duck.

Polymorphisms in the growth hormone (GH) gene that is associated with the growth rate of duck have been the target of many breeding programmes. Molecular market selection has been an acceptable tool in the acceleration of the genetic response of desired to improve production performance in livestock. Growth hormone (GH) has been considered as a candidate gene for growth traits. In this study, polymorphisms of the GH gene were evaluated for associations with body weight in 210 Bayang duck. The objective of this study was to assess the association of polymorphisms in Growth Hormone (GH) genes with growth in Bayang ducks. The polymorphism of the ducks GH gene from promoter region into exon 3 was researched by polymerase chain reaction and DNA sequencing methods. Fifty duck were genotyped by sequencing twenty mutations were detected in the Bayang duck GH gene. Mutation G→A were detected at position -142, 1155, 1117, 1423, and 1786. Mutation C→T were detected at position -62, 506, and 308. Mutation A→G were detected at position 160, 264, 293, 1245, and 2542. Mutation T→G detected at position 250 and mutation TT→AA detected at position 350. Mutation G→T detected at position 775 and mutation T→C were detected at position 1353, 1424, and 2973. All polymorphism were polymorphics except polymorphism T→G at position 250 was monomorphics. Mutation G→A in position 1117 and 1786 were restriction with enzyme Eco721 and TscaI. In the case GH-Eco721 three genotypes were observed, GG, GA, and AA with frequency 0.041, 0.59 and 0.369 respectively. For GH-TscaI locus the frequency were 0.034, 0.782, and 0.184 respectively. The effect of GH-TscaI polymorphism were observed on body weight in 4 to 8 week of Bayang duck.

Growth Hormone Receptor (GHR) gene play important roles in duck performances due to their crucialfunctions in growth and has been considered as a candidate gene for growth traits. This reasearch wasaimed to indification polymorphism of Growth Hormon Receptor (GHR) gene in Sikumbang Janti duckand this study used 68 blood duck. Polymerase chain reaction (PCR) and sequencing analysis wereconducted to detect SNP existence in exon 10 of GHR gene. The result of this study showed that wasfound two SNP at g.1112 A>G and g.1304 C>T with transision mutation in exon 10 GHR gene. Genotypesfound in the g.1112 A>G were AA and GG with types of Allele A and G. Genotypes found in the g.1304T>C the were TT and CC with types of Allele T and C. Polymorphism of GHR gene were polymorphicand Population of Sikumbang Janti duck in Hardy-Weinberg equilibrium. It could be concluded thatGHR gene has potential to can be marker genetic in Sikumbang Janti ducks.