Polymorphism of prolactine genes and its association with body weight in Bayang ducks, local duck from West Sumatera, Indonesia

Abstract

This study aims to determine the polymorphism of Prolactin (PRL | XbaI and PRL | DraI) genes in Bayang ducks using the PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism) technique and its association with weight of ducks aged 1-10 weeks. This study used 200 Bayang duck blood samples consisting of 102 male ducks (♂) and 98 female ducks (♀). DNA from blood samples was isolated using the Genomic DNA Purifification Kit (Promega) using protocol from the manucfacture. The DNA was then amplified using two primers with F: 5′-AAA TTC CCT CTC ACA GTT ACA-3′ and R: 5′-GAT GCA GAG ACA AGT TTC ACC-3′ and F: 5′-GAATAGAACACTTGACCCTG-3′ and R: 5′-TAGAGGAGGCAAGCATAG-3′ which produces fragments with a length of 416 bp and 566 bp. Restriction with XbaI enzymes that recognized the binding site (5-TT CTAGA-3′) resulted 3 genotypes: homozygote (+/+), heterozygote (+/−) and homozygote (−/−) with frequencies 0.455, 0.495 and 0.050 respectively and with frequency allel (+) 0.702 and frequency allel (−) 0.297. While the results of the restriction with enzyme DraI found three types of genotypes, namely (+/+), (+/−) and (−/−) with frequencies of 0.64, 0.35 and 0.01 respectively with frequency allel (+) 0.82 and allele frequencies (−) 0.18. From the results of the analysis, it was found that there was no relationship between these two diversity and weight ducks of duck.

Effect of a Polymorphism in Prolactin Gene on Some Reproductive Traits in TB Crossbred Ducks in Southern Vietnam

“…pcr aND pcr-rfLp assay A fragment of 536 bp of the PRL gene in exon 5 was successfully amplified in all duck samples and the representative electrophoresis image with the 536 bp fragment was clearly observed in the Figure 2. Similar with current study, many studies have also successfully amplified the target gene on exon 5 of the prolactin gene with various fragment sizes in ducks (Mazurowski et al, 2016;Ghanem et al, 2017;Yurnalis et al, 2019;Purwantini et al, 2020) or in chicken (Rashidi et al, 2012;Mitrofanova et al, 2017;Perdamaian and Daryono, 2020;Rohmah et al, 2022). As shown in Figure 3, polymorphism at the PRL/Pstl with two alleles (C and T) and three genotypes (CC: 536 bp; TT: 406/130 bp and TC: 536/406/130 bp) was clearly distinguished.…”

The association of prolactin gene polymorphism with egg production traits in Alabio and Mojosari ducks

“…This was in line with the result of Wang et al (2011) that found C and T alleles were dominant in F1 stock of China native ducks. Meanwhile Yurnalis (2019) reported that C and G al- In this study indicated that the population of Alabio was monomorphic for SNP C-5796A and T-5817C due to C allele frequency in SNP C-5796A and T on SNP T-5817C are both 0.99. Moreover, the heterozygosity of both Alabio and Mojosari ducks are in low category: 2.06% and 9.50% (SNP 1 & SNP 2), respectively.…”

Genetic Diversity of the Prolactin Gene in Three Indonesian Ducks

“…This result was in line with Wang et al [5] that found C and T alleles were dominant in the F1 stock of China native ducks. Yurnalis et al [9] reported in different SNP, PRL|DraI mutation, the C allele (0.80) was higher than the T allele (0.20) in female Bayang ducks. In an enormous population, a polymorphic site is considered an SNP if it has less than 99 percent allele frequency or less than 95 percent in the small population [19].…”

“…Previously, studies on the PRL gene had been widely carried out in Chinese duck, Khaki Campbell Ducks, and Italian duck [5,7,8]. In addition, the study of the PRL gene was also conducted in several breeds of Indonesian local duck, i.e., Bayang, Tegal, Magelang ducks [9,10]. Li et al [11] reported mutation T1326C in the intron 1 region had a positive association with egg weight in Gayou duck.…”