In order to investigate the fermentation process of Artemisia polysaccharides, this paper showcases an investigation into the effects of fermentation time, fermentation temperature, strain inoculum, Artemisia annua addition, and shaker speed on the polysaccharides production of Artemisia annua. The yield of Artemisia polysaccharides content was determined based on the optimization of single-factor test, and then a response surface test was conducted with temperature, inoculum, and time as response variables and the yield of Artemisia polysaccharides as response values. The fermentation process was then optimized and the antioxidant activity of Artemisia polysaccharides was monitored using DPPH, ABTS+, OH, and total reducing power. The optimum fermentation process was determined by the test to be 5% inoculum of Aspergillus niger, temperature 36°C, time 2 d, shaker speed 180 r/min, and 4% addition of Artemisia annua, and the extraction of Artemisia polysaccharides was up to 17.04% by this condition of fermentation. The polysaccharides from Artemisia annua fermented by Aspergillus Niger had scavenging effects on DPPH, ABTS, and OH free radicals.
Objective: Studying new carrier materials to solve the problems of bioavailability and side effects of drugs in the body and choose the suitable carrier to coat the drug. This carrier not only improves the release and absorption of drug molecules in the body, but also significantly improves the bioavailability and reduces the toxic side effects of high concentrated drugs. Therefore, this paper focuses on the research of the synthesis of a new carrier material. Methods:Using ferric nitrate as iron source, first add ferric nitrate, 1,3,5-benzene tricarbonic acid, chitosan solution and deionized water pro rata, then synthesize the product using mechanical stirring, and purify and dry the product. Results:The maximum yield of chitosan is obtained when the concentration of chitosan is 1% using mechanical stirring method, and the yield is 0.6330 g. Conclusion:The results show that the thinner the concentration of chitosan solution is, the better synthesizes the chitosan@MIL-100(Fe) and the higher the yield is. And it reaches the best result when the concentration is 1%.
The aim of this study was to investigate whether the neuroprotective effects of astragaloside IV (AS-IV) against hydrogen peroxide (H2O2)-induced damage on human neuroblastoma cell line (SH-SY5Y) are associated with synaptic plasticity. The concentration screening of AS-IV and H2O2 on SH-SY5Y cells and the protective effects of AS-IV on SH-SY5Y cells under H2O2 stress were all determined by MTT assay. The expression of postsynaptic density 95 (PSD-95) and growth-associated protein 43 (GAP-43) were measured by western blot (WB) and inmunofluorescence staining assay under the same treatment conditions. According to the MTT results, the concentration of H2O2 at 50 μmol/L for 3 h was used for the cell damage model, and various concentrations of AS-IV (0.1, 0.2, 0.3, and 0.4 μmol/L) were used to affect SH-SY5Y cells. The MTT results showed that pretreatment of SH-SY5Y cells with AS-IV (0.1, 0.2, 0.3, and 0.4 μmol/L) attenuated the damage induced by H2O2 (50 μmol/L, 51.62% cell viability) and increased cell viability to 64.19, 63.48, 65.86, and 65.81%, respectively. Western blot analysis and immunofluorescence staining showed that the protective effects of AS-IV against SH-SY5Y cell damage caused by H2O2 resulted in reduced expression of PSD-95 and increased expression of GAP-43 in comparison with the H2O2 treatment group. The conclusion shows that AS-IV protected SH-SY5Y cells and enhanced their viability under H2O2 stress. AS-IV may facilitate presynaptic and postsynaptic plasticity to exert protective effects against oxidative damage of SH-SY5Y cells.
Artemisia annua is an annual herb, which is a traditional Chinese medicine in China. The high water soluble artemisinin derivative extracted from Artemisia annua is Artemisia annua water extract. In this experiment, the modeling method is used, and the Artemisia annua water extract is used alone or in combination with other lipid-lowering drugs. Finally, the results showed that the effect of the combination of drugs was better than that of Artemisia annua water extract alone, and the effect of the drug ratio of 1:1 was the best. It can significantly reduce the content of triglyceride in serum and cholesterol, increase the ratio of H/L, and show a good synergistic effect.
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