The preparations of microbial origin inactivated by irradiation on the “Researcher” gamma device were used as potential antiradiation drugs: E. coli strain “KV-1”, “PL-6”. The preparations were obtained by growing cultures in mesopatamia broth in a thermostat at 37 °C for 3 days, then centrifuged at 3000 rpm for 40-50 min, the supernatant was decanted, the precipitate was diluted with distilled water according to the L.A. turbidity standard. … Tarasevich up to 1 billion / ml. From the grown cultures, smears were prepared and stained according to Gram to determine the purity and species of the grown culture. The prepared suspension was poured into sterile vials of 10, 50, and 100 ml each, sealed with rubber stoppers and rolled in with aluminium caps, marking with the indication of the strain, radiation dose and date. Irradiation of microbial material was carried out on a gamma device “Researcher”, a source of 60Co, exposure dose rate 3.7 kGy / h, in the range of absorbed doses from 7.5 to 30 kGy with inter-dose intervals of 2.5 and 5 kGy. Studies to determine the radioprotective effectiveness of strains of microorganisms killed by gamma-irradiation were carried out on outbred sexually mature white mice with a live weight of 18-20 g, divided according to the principle of analogues into groups of 5 animals each according to the following scheme: irradiation + E. coli strain “KB- 1”, irradiation + E. coli strain “PL-6”, control of irradiation, biological control. Acute radiation sickness was simulated using the Puma gamma device with a 137Cs radioactive source at a dose of LD80-100 / 30. The test preparations were injected subcutaneously in a volume of 0.2 cm3 three days after radiation exposure. It was found that the introduction of cultures of microorganisms inactivated by gamma-irradiation E. coli strain “KV-1”, E. coli strain “PL-6” 3 days after external radiation exposure contributed to the preservation of 60 to 80% of irradiated white mice.

Currently, despite the improvement of radiation safety measures, there is a risk of increasing frequency of radiation accidents and various disasters accompanied by explosions, fires and emissions of radionuclides. Emergencies (emergencies) of peacetime and wartime, especially accidents at radiation-hazardous facilities, including nuclear power plants, carry the danger not only of irradiation of people, but also at the same time thermal burns, mechanical injuries, chemical poisoning by harmful gorenje products, as well as other lesions. Experimental modeling of acute radiation and burn disease was carried out both separately and in combination. It was found that external total gamma irradiation of white rats at a dose of 7.5 Gy causes severe radiation sickness with characteristic clinical signs and high mortality of irradiated animals. A heat stroke simulation was performed by applying a metal plate heated to 190°C with an exposure time of 5 and 8 seconds. It turned out that the degree of burn depends on the time of contact with the surface of the body: its application within 5 seconds caused a burn of the III-A degree, an 8-second exposure of the IIIB degree, which was confirmed by the timing of formation, rejection of eshar and complete healing of the thermal lesion. The aggravating effect of ionizing radiation on the course and outcome of combined radiation-thermal pathology is demonstrated.