Obtaining radioactivated strains of microorganisms and studying their antiradiation efficiency

Abstract

The preparations of microbial origin inactivated by irradiation on the “Researcher” gamma device were used as potential antiradiation drugs: E. coli strain “KV-1”, “PL-6”. The preparations were obtained by growing cultures in mesopatamia broth in a thermostat at 37 °C for 3 days, then centrifuged at 3000 rpm for 40-50 min, the supernatant was decanted, the precipitate was diluted with distilled water according to the L.A. turbidity standard. … Tarasevich up to 1 billion / ml. From the grown cultures, smears were prepared and stained according to Gram to determine the purity and species of the grown culture. The prepared suspension was poured into sterile vials of 10, 50, and 100 ml each, sealed with rubber stoppers and rolled in with aluminium caps, marking with the indication of the strain, radiation dose and date. Irradiation of microbial material was carried out on a gamma device “Researcher”, a source of 60Co, exposure dose rate 3.7 kGy / h, in the range of absorbed doses from 7.5 to 30 kGy with inter-dose intervals of 2.5 and 5 kGy. Studies to determine the radioprotective effectiveness of strains of microorganisms killed by gamma-irradiation were carried out on outbred sexually mature white mice with a live weight of 18-20 g, divided according to the principle of analogues into groups of 5 animals each according to the following scheme: irradiation + E. coli strain “KB- 1”, irradiation + E. coli strain “PL-6”, control of irradiation, biological control. Acute radiation sickness was simulated using the Puma gamma device with a 137Cs radioactive source at a dose of LD80-100 / 30. The test preparations were injected subcutaneously in a volume of 0.2 cm3 three days after radiation exposure. It was found that the introduction of cultures of microorganisms inactivated by gamma-irradiation E. coli strain “KV-1”, E. coli strain “PL-6” 3 days after external radiation exposure contributed to the preservation of 60 to 80% of irradiated white mice.

Study of radiation-thermal damage in white rats

“…The first case of mortality in white rats exposed to radiation and heat injury was observed at day 4, while in the monoexposure (irradiation control) group it was not until day 9. Despite absolute mortality in the compared groups, mortality periods were 2-3 times longer (17, 17, 18, 24, 25 days) in the irradiated and heat-treated animals than in the control group (6,7,8,9, and 38 days) with ALE 18.3 and 12.0 days, respectively. In animals of groups 2 and 4 treated with purified turpentine, in contrast to controls, the course of radiation and burn disease proceeded according to a modified (lightened) variant.…”

“…This is exemplified by the bombing of well-known Japanese cities and the events at the Chernobyl and Fukushima nuclear power plants when, in addition to ionizing radiation, people and animals were exposed to other damaging factors, such as light and blast waves, thermal factors, etc., causing combined radiation injuries, including thermal-radiation injuries (TRI), in the emergency situations created. The course of such pathological processes has its own peculiarities, so the treatment of burns received against the background of external gamma-irradiation is very relevant [6].…”

Development of an Optimal Model of Combined Radiation and Biological Lesions

“…Radiation action on the organism inhibited bone marrow hemopoiesis and emptied central and peripheral immune organs, causing the growth of autoimmune processes. The therapy of acute radiation disease is a preparation based on chemical and biological substances [ 5 – 8 ].…”

“…e therapy of acute radiation disease is a preparation based on chemical and biological substances [5][6][7][8].…”

Development of a complex anti-radiation protective drug

“…IOP Conf. Series: Earth and Environmental Science 935 (2021) 012024 IOP Publishing doi:10.1088/1755-1315/935/1/012024 2 E. coli PL-6 and B. bifidum 1 in the process of vital activity produce antibacterial substances, enzymes, antigens, entero-and exotoxins, cytokines, which individually and in combination with each other have radioprotective properties [10].…”