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Chlorophyll a concentration, the total abundance of bacteria, the number of bacterial cells with active metabolism, and the abundance of saprotrophic bacteria were studied in the surface and bottom water layers of Lake Beloe in winter and spring 20152016. The abundance of sanitary-indicator microorganisms was determined for the first time with the use of Petrifilm test-systems (3MTM Petrifilm). In most cases, Lake Beloe water in spring and winter was found to correspond to eutrophic level (in terms of chlorophyll a concentration in water) and polysaprobic status (in terms of microbiological indices). By its sanitary-microbiological characteristics, the lake is clearthe values of the total microbial count, determined with the use of test-systems 3M Petrifilm Aqua (AQHC), were 1000 CFU/mL, and the abundance of coliform bacteria, determined with the use of test-systems 3M Petrifilm Aqua (AQСC), varied from 20 to 135 CFU/100 mL. Coliform bacteria were mostly found in the bottom water layer. It was shown that, in the organization of ecologicalmicrobiological studies, special attention is to be paid to the bottom horizons of water bodies.