Sarcoidosis is epithelioid cell granulomatosis of unknown etiology. All the schemes of its treatment are of a recommendatory nature. Methotrexate (MTT) is considered a second-line drug in the treatment of sarcoidosis.Methods. A retrospective observational study of patients with progressive sarcoidosis (n = 104), treated with MTT once a week, was carried out. Clinical, laboratory, functional and radiation parameters were evaluated with an interval of 3 months ≤ 1 year.Results. The use of MTT was accompanied by an improvement in the radiation picture (64.1% by the end of the year), an improvement not only in spirometry parameters, starting from the 3rd month (54.7%), but also in the general condition of the patients (63.8% – at the 6th month). Adverse reactions with drug withdrawal were most often observed (15.4%) during the first 3 months, and subsequently their frequency decreased.Conclusion. According to the results of the study, it was shown that methotrexate can be recommended for further use in progressive sarcoidosis, as well as in patients, who have previously received systemic glucocorticosteroids.

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Neurosecretory cells of the main zones of the amygdaloid complex were studied by Golgi method and light and electron microscopies. Light microscopy demonstrated that these cells have long axons and few branches and possess the properties of karyochrome and light cells. Key Words: brain amygdaloid complex; neurosecretory cells; neuronal and electron microscopic organization of the brainNeurons with morphological signs of secretory activity were found in the amygdaloid complex (AC) of rat brain. The goal of this study was to characterize these cells using light and electron microscopies and to demonstrate their identity to long-axon and low branching neurons (according to classification [6]). MATERIALS AND METHODSExperiments were performed on adult male and female Wistar rats weighing 300-320 g. The material was taken using a MBS-9 lens, treated as described elsewhere [4], and fixed in cold 2.5% glutaraldehyde in phosphate buffer. Slices were prepared in an LKB ultratome, contrasted with lead citrate [15], and examined under a JEM 2000 EX transmission electron microscope. RESULTSNeurosecretory cells are found in 2 main sexual dimorphism M zones (the dorsomedial and anterior cortical nuclei) described previously [2,5].Light microscopy and Nissl staining with cresyl violet revealed 3 types of neurons: karyochromic, cytochromic, and light cells. Karyochromic neurons are generally large or middle-sized cells with polygonal bodies, large dark-stained nucleus occupying the most part of the cell body, and practically homogeneously dark-stained cytoplasm. Cytochromic neurons are larger Department of Human and Animal Morphology and Physiology, Bashkirian State University, Ufa than karyochrome neurons and have round bodies, basophilic cytoplasm, and light nucleus with large nucleolus. These cells are designated as cytochromic neurons because their staining is determined by specific features of the cytoplasm. Light neurons have round or oval middle-sized bodies, light perikarya containing small lumps and granules of basophilic substance, and light euchromatin-rich nuclei. This classification of neurons based on tinctorial properties of Nissl-stained cells is similar to that proposed previously [8], but differs by clearly defined meaning.The dorsomedial nucleus are formed by karyochromic and light neurons, while the anterior cortical nucleus contains all types of neurons.Studies of the neuronal organization of these AC zones showed that long-axon and low branching neurons with short dendrites are prevalent in the dorsomedial nucleus. Bodies of short-dendrite neurons lie on the vascular walls, and their processes surround the vessels (Fig. 1, a). Short-dendrite neuron network with included pyramidal and spindle-like neurons of the cortical type is found in the anterior cortical nucleus, especially in the medial part of the surface cellular zone. Comparison of Nissl-stained and Golgi-impregnated specimens showed that short-dendrite neurons are karyochromic cells, while cytochromic cells correspond to pyramidal and spindle-like n...