The article explores the impact of microbial biologics on the yield of one-year ryegrass and the quality characteristics of feed obtained during the silage of green crop mass. The introduction of preparations, made on the basis of live strains of microorganisms – Bacillus subtilis (“Naturost”), Lactobacillus buchneri (“Naturost-Aktiv”), and Bacillus megaterium (“Naturost-M”), was carried out by soaking the seeds before sowing and processing vegetative organs of plants. Small-scale field studies were conducted in the Vologda Oblast in 2019–2020, and silage experiments were held in a laboratory. Under the influence of biologics, the productivity of ryegrass green mass has increased up to 41.2 %. Moreover, biologics had an impact on the preservation of nutrients in the produced silage, the yield of feed units, and exchange energy in ryegrass silage mass from the seeding area of the experimental variants was higher, compared to the control, by 36.2–52.8% and 31.3–42.8%, respectively. A high yield of green crop mass was obtained in experiments using the “Naturost-Aktiv” and “Naturost” biologics. In terms of physical and chemical parameters and nutritional value, the best feed was obtained from ryegrass biomass in the variant with a preparation based on Bacillus subtilis (“Naturost”) bacteria.