Hedysarum grandiflorum samples were collected from 7 natural populations of the Volgograd, Samara and Lugansk People's Republic and the features of seed reproduction were studied under ex situ and in vitro conditions. The optimal temperature for seed germination and the dependence of germination and seed vigor on the storage were revealed. We observed the maximum germination rate in the scarified seeds of H. grandiflorum of 2018 collection and it was equal to 70%.It was found that the seed germina-tion and seed vigor are low and quickly decrease with years of storage, while their scarification allows to increase these indicators. It is noted that the relatively low germination of the studied seeds is leveled by the rather high productivity of species in general. The optimal mode of sterilization of H. grandiflorum seeds was experimentally determined - 10% Lysofornin® 3000 solution for 5 minutes. The percentage of sterile seedlings was 60%.

The application of isolated plant tissue and organs is getting more and more actual along with traditionalplant ex situ conservation methods. The peculiarities of hormon regulation of morphogenesis have been established fora Hedysarum razoumovianum cultivation. Optimal concentrations of growth regulators for stages of induction of explantdevelopment, microshoots regeneration and their rooting have been chosen. The development of reproduction plantsmethods is the basis of gene pool preservation.

Despite more than a century of research on effective biotechnological methods to reproduce various plant species, microclonal reproduction continues to be an important tool for large-scale production. The clonal seedlings of important species maintain genetic fidelity and do not contain pests. In some cases, microclonal propagation is the only method that contributes to the maintenance and economic value of specific agricultural plant species. Microclonal reproduction as a method has solved many phytosanitary problems and has allowed both expansion and access to high-quality plants for producers from different countries and economic conditions, thus effectively contributing to the expansion of agriculture now and in the foreseeable future. Currently, this method is widely used in the creation of planting material for crops for agriculture and cultivation of crops of industrial floriculture, fruit, berry crops and woody plants. Thanks to this method, it is possible to create in vitro banks of rare and valuable plant genotypes. Modern technologies of clonal micro-multiplication are at the stage of industrial flow, which quickly responds to market demands. The analysis of domestic and foreign sources of scientific research on microclonal plant propagation has shown that, at the present time, the cost of its use is quite high and requires the presence of laboratories with appropriate equipment and highly qualified staff. Modification and adaptation of the method of microclonal reproduction of plants contributes to the implementation of the morphogenetic potential, determines the specific features of the source material, the type of explant, its physiological state, the composition of nutrient media, and cultivation conditions. At the same time, the cultivation of healthy plants will significantly increase the yield of valuable agricultural products and high adaptive properties of healthy plants that allow them to be cultivated with less chemicals, which will significantly increase their nutritional value and give a greater opportunity to obtain organic products with high-quality characteristics.

No abstract