Introduction Iris L. are promising in medicine due to the biological activity of extracts. Iris sibirica L. is spread in Russia but its phytochemical composition has not been studied in detail though it is included in the Red Book. For this reason, I. sibirica L. biotechnology is in high demand. One of the key points in biotechnology is the regulation of plant metabolism using phytohormones. Obtaining of chromatographic metabolite profiles allows to control this process. Objective The aim of this study was to develop an approach for effective control of biotechnological raw materials of I. sibirica L. by flavonoid profiles using high‐performance liquid chromatography–tandem mass spectrometry (HPLC–MS/MS) and to investigate the influence of phytohormones in nutrient media on content of flavonoids. Methodology Iris sibirica L. regenerated plants were grown on Murashige–Skoog media with 6‐benzylaminopurine (6‐BAP) and α‐naphtylacetic acid (NAA) additives. To optimise extraction conditions, the design of the experiment was used. Profiles of polyphenols were obtained by HPLC–MS/MS in the positive and negative ionisation modes. Results The process for efficient extraction from leaves of I. sibirica L. were developed. The factors influencing the extraction efficiency of flavonoids have been determined. A total of 36 compounds were identified by HPLC–MS/MS. Among them isoflavones and their glycosides are the main classes. Addition of an auxin‐like hormone increased the non‐polar flavonoid levels, but decreased the polar ones. The variation in concentration of cytokinin (6‐BAP) affected almost all of the analytes. Conclusion The methodology for effective control of I. sibirica L. raw plant material biotechnology was developed by analysing obtained chromatographic polyphenol profiles.

Despite the plant's extensive area of distribution, Potentilla alba L. natural resources are scarce and cannot meet the modern needs of the pharmaceutical industry. Because of the mass preparation of medical raw materials by using P. alba, it entered into the list of rare and endangered species plants of the Red Data Book of the Republic of Belarus. This plant is not represented in the wild flora of Western Siberia, but there is a great need for developing a method for the mass propagation of P. alba using in vitro culture in order to obtain a high-quality planting material. At the explant stage, the technique of the P. alba introduction into in vitro culture is developed. This paper reveals the morphogenetic features of the development of P. alba explants of different types and the regenerative capacity of the tissue culture. At the micropropagation stage, the optimum culture media and the growth conditions for the regenerated plants are selected. At the stage of test-tube plants rooting and transferring them into ex vitro conditions, the most effective means of adaptation to non-sterile conditions in hydroponics are proposed.

Methods of biotechnology allow to obtain high-quality medicinal plant raw materials in a short time, in large quantities without destroying natural reserves. Biotechnological approaches such as aeroponic technologies have the potential for large-scale cultivation of iris plants and production of secondary metabolites. Microclonal reproduction makes it possible to obtain a healthy planting material in the required amount, regardless of the time of year. The combination of these two technological approaches will allow to develop biotechnology of year-round production of medicinal plant raw materials of Siberian iris. The study determined the content of 6-benzylaminopurine on the stage actually micropropagation for the formation of the greatest number of adventitious shoots of optimal length. The required content of BAP in the nutrient medium for I. sibirica was 2.5–5.0 µM. The introduction of cytokinins in the nutrient medium together with auxins, L-glutamine and adenine sulfate 100 mg/l, as well as the alternation of low and high concentrations of cytokinin enhanced the regenerative effect of BAP. With year-round cultivation of regenerative plants in aeroponic conditions, the amount of biomass of plant raw materials I. sibirica for this method was about 31.2 kg / m2 of crude weight in one year. It is established that intact plants and regenerative plants I. sibirica, obtained on the basis of the developed biotechnology, had identical group composition of biologically active substances. It is revealed that the sum of flavonoids in the leaves of hydroponic iris plants exceeded the content in the leaves of intact plants by 3 times, and the content of essential oil in regenerate plants and hydroponic leaves of the Sterch variety but higher by 26% compared with the leaves of intact plants. Aqueous and alcoholic extracts of I. sibirica showed antiviral activity against herpes virus. With low toxicity, both intact plants and regenerative plants had a relatively high selectivity index.

The raw materials Iris sibirica L. were obtained by biotechnological methods. The relationship between biomass accumulation and a mangiferin content is peculir to Siberian iris. On a medium with 5.0 μM BA supplemented with auxin, the xanton content in the phytomass sharply decreased with an increase in the total shoot height. To maintain a balance between biomass accumulation and a mangiferin content in I. sibirica, it is recommended to use media with 2.5 μM BA supplemented with auxin. Water and ethanolic extracts of I. sibirica showed a high antriviral activity against herpes simplex virus of type 1.

Stellera chamaejasmae L., family Thymelaeaceae, is a herbaceous p~rennial found in the USSR in Eastern Siberia [1]. Its chemical composition has not been studied previously [2]. W~ hw investigatPd the composition of the coumarins of the roots of S__ chamaejasmae L. growing in the Mongolian People' s Republic. The material was collected by the resources team of a combined Soviet-Mongolian comprehensive biologicial expeditionof the Academy of Sciences of the USSR in the gorge of the river Ts~ts~rl~g in the Khangai range (Arkhangaiskii aimak, Ts~ts6rl~g somon) in June, 1972 in the flowering period of the plants. Five coumarin derivatives were detected by the chromatography (FN-12 paper with petroleum ether as the mobile phase and ethylene glycol as the stationary phase) of an ethanolic extract of the roots of S. chamaejasmae