No abstract

No abstract

A population of ethnic Kyrgyz was examined with a view to elucidating the relationship between the adiponectin G276T gene polymorphic locus and the development of abdominal obesity (AO). The study included 288 subjects at the age between 40 and 70 years. 139 of them (81 women and 58 men) presented with AO while 149 without obesity (62 women and 87 men) constituted the control group. The measured anthropometric parameters included arterial pressure, blood glucose, insulin, and leptin levels, blood lipid composition. Genotypes of adiponectin (AN) G276T gene polymorphism were identified by means of PCR-RFLP analysis. The relationship between the presence of the adiponectin G276T gene polymorphic and the development of abdominal obesity in the women was demonstrated. Specifically, 31% of the women with AO were carriers of T allele compared with 17% in the control group (χ2=7.89; p =0.005). The GT + TT genotype and carriage of T allele were associated with an increased risk of development of abdominal obesity (OR=2.5; 95% CI = 1.25-4.97 for the genotype and OR = 2.2; 95% CI = 1.26-4.000 for the allele). No such relationship was documented among men. The women with AO and GT+TT genotype more frequently than homozygotes presented with type 2 diabetes mellitus (64 and 37% respectively; p=0.017), hypertriglyceridemia (41 and 16.2% respectively; p=0.016), and enhanced blood glucose level (7.74±3.3 and 6.52±1.17; p=0.033). Moreover, their HOMA index was higher than in the homozygotes (3.5±1.7 and 2.63±1.24 respectively; p=0.02). It is concluded that the adiponectin G276T gene polymorphic variant in the women of Kyrgyz ethnicity is associated with abdominal obesity, type 2 diabetes mellitus, hyperglycemia, and hypertriglyceridemia.

Цель исследования - оценить вклад полиморфных вариантов g.15661G> T (ген ADIPOQ), p.A222V (ген MTHFR), p.Q192R (ген PON1), p.K23E (ген KCNJ11), g.53341C>T (ген TCF7L2), p.V109D (ген ITLN1) и p.P12A (ген PPARG) в развитие ожирения для лиц кыргызской национальности. Исследование было проведено по типу «случай-контроль» и включало 130 пациентов с ожирением (мужчин - 65 (50,0%), женщин - 65 (50,0%)). Группа сравнения - 115 человек без ожирения, метаболических нарушений и сердечно-сосудистых заболеваний в анамнезе (мужчин - 62 (53,9%), женщин - 53 (46,1%). Генотипирование осуществляли методом ПЦР-ПДРФ. Анализ межгенных взаимодействий проводился с использованием программы MDR 3.0.2. Маркерами развития ожирения в кыргызской популяции являются аллель V и генотип VV локуса p.A222V (MTHFR), а также аллель K и генотип KK локуса p.K23E (KCNJ11). При наличии генетического профиля AV (p.A222V, MTHFR) / EK (p.K23E, KCNJ11) вероятность развития ожирения возрастает более чем в 3 раза, ОШ=3,49, 95% ДИ=[1,44-8,45], p=0,001. In this study, we investigated whether polymorphisms g.15661G>T (ADIPOQ), p.A222V (MTHFR), p.Q192R (PON1), p.K23E (KCNJ11), g.53341C>T (TCF7L2), p.V109D (ITLN1) and p.P12A (PPARG) are associated with obesity in the Kyrgyz population. We genotyped 245 nonrelated adults Kyrgyz individuals. 130 patients (male - 65 (50,0%), female - 65 (50,0%) with obesity and 115 non-obese control subjects (male - 62 (53,9%), female - 53 (46,1%). Genotyping of single-nucleotide polymorphism was performed by PCR-RFLP method. Analysis of intergenic interactions conducted with MDR v.3.0.2 program. Тhe V allele and the VV genotype of the p.A222V locus (MTHFR), as well as the K allele and the KK genotype of the p.K23E locus (KCNJ11) increase risk of obesity in the Kyrgyz population. Subjects having the VV genotype of p.A222V locus (MTHFR) and KK genotype of the p.K23E locus (KCNJ11) had 3-fold [OR = 3,49 (1,44-8,45); p = 0,001] higher risk of developing compared with subjects carrying neither of these genotypes.

No abstract