The aim of the work was an investigation of the effect of hydrocarbons on bacterial and fungal cenoses of sod-podzolic soils on the territory adjacent to the oil storage in the village Malye Kolpany, Leningrad Region. NGS methods were used to analyze the taxonomic composition and structure of the bacteriocenosis and the method of direct microscopy by Demkina-Mirchink to determine the length of the fungal mycelium and the number of spores. Taxonomic and structural changes in the bacterial community led to the dominance of genera containing numerous species-oil destructors. It is established that the main role in the decomposition of oil in the investigated territorybelongs to prokaryotes related to the genera Pseudoxanthomonas, Methylobacterium and Nocardioides. Mycocenosis showed high sensitivity and low adaptability to oil contamination.

Anthropogenically altered soils of Saint Petersburg and Luga (Leningrad Region) were investigated for the presence of thermophilic aerobic chemoorganoheterotrophic bacteria, potentially capable of decomposing hydrocarbons at elevated temperatures (60 C). 6 strains of pure spore-forming cultures of bacteria were isolated. Analysis of the nucleotide sequences of the 16S rRNA genes showed that they belong to the genera Geobacillus and Aeribacillus. For the first time, we obtained information on the presence of representatives of the genus Aeribacillus, which are typical inhabitants of hot springs and zones with geothermal activity, in the soils of the regions of Saint Petersburg and the Leningrad Region.