The use of liposomal nanoparticles with an incorporated active substance is an innovative and promising approach to diagnostics and therapy. The application of liposomal nanoparticle-based drugs allows for targeted localized delivery, overcomes the natural barriers within the body effectively, and minimizes possible side effects. Liposomes are able to contain a variety of ingredients with practically no limitations to their chemical composition, chemical properties, or size of constituent molecules. This study evaluated the ability to control the passage of fluorescent dye-filled liposomes through the intestinal mucosal barrier after oral administration. For this purpose, the increase in transcutaneous registered fluorescence from tetrabromofluorescein dye was recorded and analysed. Fluorescence intensity was measured at the proximal end of the tail of an animal model after oral administration of the liposomes. Measurements were taken at the excitation wavelengths of 365 and 450 nm. The fluorescence intensity in the group treated with the fluorescent contrast agent encapsulated in liposomal particles increased 140% of the initial level, but in the group treated with pure contrast agent, the increase in detected fluorescence intensity did not exceed 110%. Mice that received empty liposomes as well as the control group did not demonstrate statistically significant changes in fluorescence intensity. A potential application of our results is an express laser optical method of monitoring the transport of orally administered liposomal particles. The results can be used to help create new optical tools for use in the development of new drugs and in high-throughput screening used during their testing.

No abstract

Local hemodynamic parameters were studied by means of laser Doppler flowmetry in 15 patients with psoriasis in the stationary stage, who have plaques on the inner surface of the forearm. LDF signals recorded at the site of psoriatic lesions of the tissue as well as in the intact tissue at a distance of 1-2 cm from the affected area were analysed. LDF signals were postprocessed by continuous wavelet transform using the Morlet wavelet.

invasive control of influence of polyethylene glycol on transport function of fluorescent colored liposomal nanoparticles,"

The purpose of this work is to diagnose microcirculatory disorders in the tissues of psoriatic plaques in patients with psoriasis. Material and methods. Using the method of laser Doppler flowmetry (LDF), local hemodynamic parameters were studied in the place of psoriatic lesion of tissue and in uninvolved skin. Results. In the psoriatic plaque, there is an increase in average perfusion, myogenic and cardiac fluctuations, as well as nutritive blood flow as compared to the area of intact skin. Conclusions. The use of spectral analysis of the LDF signal has great potential for assessing the contribution of each link of the microvascular bed in the study the pathogenesis of psoriasis and evaluating the effectiveness of the therapy.