HLA molecules are expressed in almost all nucleated cells of the body. These molecules are extremely variable and responsible for tissue specificity recognition. Precise HLA typing is crucial for tissue and organ transplantation. Usually, HLA‐typing NGS methods are based on the assignment of reads to a certain previously reported HLA allele presented in the IPD‐IMGT/HLA Database. But there is a limited number of tools able to identify novel alleles that have not yet been reported and thus absent in the database. Such alleles carry mismatches distinguishing them from all known alleles in the database. Therefore, manual evaluation of the identified HLA alleles in the genome browser is a compulsory step in the analysis, and one that is labor intensive and time consuming. We present the development and validation of a freely available web‐application for the identification of novel HLA alleles in the most relevant HLA class I and II genes from NGS data. The tool can also be used for automated data quality assessment. The software was validated by analyzing 330 alleles. The results are concordant with orthogonal methods.

We have observed that when 2-thiomorpholino-5-trifluoromethylbenzaldehyde is reacted with barbituric acids under Knoevenagel condensation conditions, a novel heterocyclic system is formed: 1,2,4,4a,5,6-hexahydrospiro [[1,4]thiazino[4,3-a]quinoline-5,5'-pyrimidine]-2',4',6'-triones 1a,b, i.e., two new C-C bonds are formed during the reaction. The reaction occurs through the o-vinyl derivatives 3 [1, 2] followed by their cyclization according to a tert-amino effect mechanism [3,4]. When monosubstituted barbituric acids were used, two isomers could be formed. We have shown that with monosubstituted barbituric acid 2b, a 1:1 mixture of spiro-linked condensed [1,2-a]quinolines 1b is formed in 78% yield. One of the isomers can be isolated in 33% yield by fractional crystallization from aqueous alcohol.

No abstract

No abstract

Genomic full‐length sequence of the HLA‐B*44:348 allele was identified by next generation sequencing in a Russian individual.