NK cells lyse virus-infected cells by degranulation; however, alterations in NK cell degranulation in persistent viral infections have not been directly studied. Earlier reports have documented a decrease in NK activity in patients with frequently recurring herpes (FRH). We corroborate these findings by showing that the degranulation responses of blood NK cells from patients with FRH, both during relapse and during remission, are significantly lower than those in healthy donors. The impaired degranulation was probably not caused by defective target cell recognition, since it was observed upon stimulation both with K562 cells and with a receptor-independent stimulus (phorbol 12-myristate 13-acetate plus ionomycin). We also show that the intracellular expression of perforin and CD107a by NK cells from patients with FRH is not different from that in healthy donors, thus excluding that the low NK cell degranulation in FRH is caused by a smaller size of the lytic granule compartment. We confirm previous reports on lowered NK activity in FRH patients and show that NK activity is significantly impaired only during remission, but not relapse; the causes for the discrepancy between the low degranulation and "normal" NK cell activity during relapse are discussed. In all, these data point at the deficit of NK cell degranulation in FRH. Whether this is a predisposing factor or a consequence of herpes simplex virus infection requires further investigation.

Национальный исследовательский центр эпидемиологии и микробиологии им. Н.Ф.Гамалеи, Москва; 2 Государственный научный центр Институт иммунологии, Москва; 3 Московский научно-практический центр дерматовенерологии и косметологии; 4 Московский НИИ эпидемиологии и микробиологии им. Г.Н. Габричевского

На клинических моделях различной локализации инфек ции офтальмо , урогенитального хламидиозов и синд рома Рейтера документированы феномены: повышения иммунокорригирующей1 эффективности базового лече ния патологии за счет дополнительного проведения диф ференцированной моно , пассивной, комплексной имму нотерапии деринатом, озонированным раствором хлори да натрия, нормальным донорским гаммаглобулином, их комбинацией; преимущества многокомпонентной моду ляции перед моновоздействиями и изменение мишеней фармако немедикаментозных корректоров при их комби нации.

We studied the state of antiviral defense in patients with severe course of herpetic infection of anogenital and labial localization and the frequency of its combination with other herpes virus infections. It was found that severe course of herpetic infection caused by herpes simplex virus occurs against the background of combined secondary immunodeficiency and its complication. We first demonstrated that severe course of the disease is associated with mixed viral infection.

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