The spermatogenic epithelium Spermatogenous cells Interstitial tissue (the interstitium) a b s t r a c t Introduction: The necessity of studying the peculiarities of the structural organization of the testes is determined by its participation in performing important functions for the body e making spermatozoa and the production of male sex hormones. Despite the presence of works devoted to the study of the peculiarities of the structural organization of the testes, many questions remain unresolved or require clarification. Materials and methods:As a biological test object used white Mature rats male Wistar rats at the age of 2 months. Seminal glands of male rats were used as a trial material for study. Tissue samples was investigated using a digital microscope Axio Imager.M2 (ZEISS, Japan) with software for image analysis AxioVision SE64 Rel. 4.8.3 and ZEN 2011. When sightseeing microscopy studied morphological features of the structure of the testes, and then determined their morphometric parameters. Results: Found that in rats-males aged 2 months in the testes, the proportion of interstitial tissue to the area of convoluted seminiferous tubules is about 1:30. It is shown that in the period of puberty of male white rats the highest percentage of the total amount of the spermatogenic cells are Mature male sex cells e spermatozoa. Second in quantitative terms are male stem cells spermatogonia. Discussion: Development and sexual differentiation of the testes is a complex process. The most important indicator of the structural and functional development of the testis is characteristic of the spermatogenic reservoir. The obtained results clearly demonstrate the maturity of experimental animals.
These studies are conducted on 36 human embroys and foetus. Four stages are marked out in the development of connective tissue of the duodenum: 1) mesenchymal stage (28-35 the day of the embryo); 2) the stage of loose embryonic connective tissue (35 days - 3.5 months of foetus); 3) the stage of formation of the definitive loose connective tissue (3,5-6,5 months of the fetus); 4) the stage of initial definitive development (6,5 months before birth of the fetus). Each stage is characterized by a specific set of cellular differens, chemical composition of polysaccarides of cells of connective tissue and intercellular substance. On mesenchymal stage of development, the cells and intercellular substance CHIC-positive substances – contain glycogen and proteoglycans that give metachromasia, stained with alcian blue on Shubitch, Hale, but CHIC are negative. At the stage of loose connective tissue in mesenchymal cells, the reduction of glycogen and accumulation of glycosaminoglycans are identified. Glycogen, hyaluronic acid and precursors sulfated groups glycosaminogly-cans are detected in fibroblasts, endothelium of capillaries, in primary blood cells, in macrophages. At the stage of formation of loose connective tissue, there is the accumulation of TIME – amelanotic compounds (proteoglycans) in the cells and in the intercellular substance. Identification of them showed that the precursors of sulfated glycosaminoglycans and chondroitin sulfates (HSC) type C are identified. The intensity of the response to glycogen decreases. In the initial definitive development in the cytoplasm of fibroblasts – there is moderate staining on Hale-positive substances, poor color on Shubich and toluidine blue. In plasmablastics and in B-lymphocytes, the glycogen and glycosaminoglycans types of hyaluronic acid are identified. In the cytoplasm of mast cells, the substances such as hyaluronic acid, HSH type and unfinished synthesis heparina are identified.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
customersupport@researchsolutions.com
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.