The characteristic of bulls-producers of red breeds of OAO Barnaulskoe breeding enterprise on the quality of sperm and its resistance to cryopreservation are presented. The breeding enterprise has examined samples of water, soil, feed for the content of heavy metals. In the studied samples, no excess of the LOC was found. The research results were processed on a PC using population statistics methods. Angler bulls produced the largest amount of ejaculates unsuitable for cryopreservation. It is 20.6%. According to this indicator, they surpassed the producers of red Danish and red steppe breeds by 4.1-7.8%. The animals of the latter breed showed the smallest amount (1.7%) of ejaculates rejected after defrost. In Angler bulls, this indicator was 5.95%, in red Danish bulls it was 2.95%, which is 3.52 and 1.75 times higher than in red steppe breeders (P <0.01-0.001). Significant differences were established between individual bulls of the red steppe breed in the number of ejaculates rejected before cryopreservation (2.84-22.29%) and after defrost (0-7.09%). The differences between breeds in the frequency of rejected ejaculates before freezing and after cryopreservation indicate a certain role of heredity in the resistance of animals for these indicators. Spearman’s rank correlation coefficient between these traits was 0.312. It indicates the possibility of preliminary assessment and selection of bulls according to the first indicator. But the final assessment of the bull-producers can be made by the frequency of rejected ejaculates after thawing. We propose to consider the issue of additional accounting in breeding programs for the resistance of the seed of bulls-producers to cryopreservation.

Results of the evaluation of copper levels in the liver, kidneys, lungs, and spleen of Landrace pigs are presented. Studies were performed on clinically healthy six-month-old animals bred in a large pig farm in Altai Krai. Animal housing conditions were standard, typical for meat-fattening feeding was used. The authors performed an elemental analysis of samples of parenchymal organs using atomic absorption spectrometry with flame and electrothermal atomization. The data were processed using Microsoft Office Excel and the R programming language in the RStudio data analysis environment version 2022.07.2+576 (RStudio, PBC). It was found that the distribution in the case of copper accumulation in the kidneys and lungs was different from usual, and the dispersions were not homogeneous. Based on the mean value and median, the authors established an increasing ranked series of the copper content in the organs: spleen < lungs < kidneys < liver, in numerical terms: 1: 1.1: 6.4: 7.5. Median copper values in the liver, kidneys, lungs, and spleen were 6.25; 5.02; 0.88; and 0.83 mg/ kg, respectively. More excellent uniformity is characteristic of copper accumulation in the lungs and spleen. Using the Kraskell-Wallis criterion, we found that the collection of copper varies significantly in the organs under study (H = 80.03, df = 3, p < 0.0001). Pairwise comparison showed significant differences for pairs: “liver - lungs” (p < 0.0001), “kidneys - lungs” (p < 0.0001), “liver - spleen” (p < 0.0001), “kidneys - spleen” (p < 0.0001). Cluster analysis revealed two groups: “lungs - spleen” and “liver - kidneys,” which differ significantly in the level of copper accumulation. The data obtained can be used to calculate the corresponding standard values of the copper content in the liver, kidneys, lungs, and spleen of Landrace pigs under the conditions of Western Siberia.

The study of fattening and meat traits of farm animals is relevant in the framework of ecological and genetic screening. The purpose of the work was to establish the infl uence of the genotype of sows on the carcass weight of the off spring obtained from them. Three groups of sows of Landrace breed were formed according to the principle of analogues, in each group they were full sibs, the total number of animals was 13. Pigs were reared in a large pig farm in the Altai Territory. The conditions of housing the animals corresponded to the standard requirements for pork fattening. At the age of about 150–160 days when fattening up to 100 kg of live weight slaughter was performed. The determination of the carcass weight was established by weighing on monorail scales for static mass estimation. The data was evaluated using Microsoft Offi ce Excel and the R programming language in the RStudio data analysis environment version 2022,07,2+576 (RStudio, PBC). The weight of the carcass characterized by the median in litters No. 2386, No. 1902, No. 2849 was 59,60, 63,60 and 71,60 kg, respectively. The analysis of variance carried out using the Kraskel-Wallis criterion indicated that the carcass weight significantly differed in the estimated litters (H = 6,36, df = 2, p = 0,041). A pair wise comparison using the posteriori Dunn test with the Hill correction indicates signifi cant diff erences in the pair of litters No. 2386 and No. 2849. Cluster analysis revealed two clusters: litter No. 2849 and litters No. 1902, No. 2386. The data obtained indicates the infl uence of pig heredity on the studied trait, and can be used in breeding programs and genetic-breeding research.

The presented results expand the understanding of the normal chemical composition of the body. The study was carried out on the basis of a pig-breeding farm in the Altai Territory in 2016-2020 on clinically healthy 6-month-old pigs of the Landrace breed. Chemical analysis of pig bristles was performed by inductively coupled plasma atomic emission spectrometry. The data were processed using Statistica 8 software (StatSoft Inc., USA). A study of the correlations between phosphorus in bristles and other macro-, microelements, as well as chemical elements associated with phosphorus, has been carried out. Only positive connections of different strengths have been established between phosphorus and elements involved in the formation of bone tissue (calcium, magnesium, manganese), copper, iron and strontium. There are two groups of direct interactions of different strengths between the chemical elements associated with phosphorus. Explained supposedly the essence of the connections, which is consistent in the bristle, as the final site of exchange, with organs and tissues of mammals.

The study of the biological role of chemical elements in farm animals is one of the issues that scientists continue to investigate. A comprehensive study of the gene pool and phenofund of breeds and species of farm animals is being carried out in Siberia. An important issue is the search for vital markers of macronutrient accumulation, including sodium in organs and tissues of animals. The article presents data on the content of sodium in the blood serum of offspring obtained from bulls-producers of the Holstein breed. The experiment was carried out at OAO Vaganovo, Kemerovo region. The sodium concentration was determined by the atomic emission method on an ICP AES IRIS spectrometer at the V. S. Sobolev Analytical Center for Collective Use of the Institute of Geology and Mineralogy SB RAS. In the area of Holstein cattle breeding, there is no excess of LOC for heavy metals in soil, water, feed, organs and tissues of animals. The influence of the genotype of the fathers of the Holstein breed on the sodium content in the blood serum of sons was established. The sodium concentration in Fabio’s offspring was 1.9 times higher (240.4 mg/ l) than in Brio’s sons (P ˂ 0.001). By the level of sodium in the serum of offspring, bulls-producers were arranged in the following order: Brio ˂ Bonaire ˂ Malstrom ˂ Fabio in a proportion of 1: 1.3: 1.5: 1.9. The phenotypic variability of this element between the offspring of different producers differed by 3 times or more. Interbreed differentiation, the influence of the genotype of producers, differences between lines and families sign the hereditary determination of resistance and susceptibility to the accumulation of macro- and microelements in organs and tissues of animals.