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This paper studied the dynamics of the blood leukocyte composition in Wistar male rats at rest and when swimming with a weight before and after being administered a succinate-containing drug (Suc, meso-2,3-dimercaptosuccinic acid). Two control groups of animals kept in standard vivarium conditions were selected: those that received Suc 12 hours before the examination (VivC+Suc) and those that did not receive it (VivC). Similarly, two groups of animals were formed that were swimming with a load (4 % of their body weight) to exhaustion: those that received the drug 12 hours before the test (Swim4%+Suc) and those that did not receive it (Swim4%). Unidirectional shifts characterizing the morphofunctional state of white blood cells under experimental conditions were detected. In VivC+Suc animals, compared to VivC, we found an increase in the number of leukocytes due to the growing number of eosinophils and monocytes, large lymphocytes and microlymphocytes with a relative decrease in the number of small lymphocytes and an unchanged level of granulocytes with a tendency towards a decrease in the number of stab neutrophils. In the Swim4%+Suc group, compared to the Swim4%, changes in the number of white blood cells and their subpopulation composition manifested themselves in a similar way to the redistribution of immunocytes identified in the control groups. The increase in the neutrophilto-lymphocyte ratio in the Swim4%+Suc group as an indicator of stress tolerance corresponded to the increase in the swimming time of rats by the factor of 2.8. The differences in the blood cell composition between the VivC+Suc and VivC groups are viewed as the influence of Suc on the body’s preparation to the fulfilment of the protective function under physical load, since the distribution pattern of the blood leukocyte composition in rats from the VivC+Suc group is very similar to that of the Swim4%+Suc group. The practical significance of this study is associated with the search for new biologically active substances that optimally influence the immune system of animals under increased load. New data on the mechanism of blood redistribution in animals under the action of Suc before the swimming test can be used to study the manifestation patterns of acute adaptation effect. For citation: Rubtsova L.Yu., Mongalev N.P., Vakhnina N.A., Shadrina V.D., Chupakhin O.N., Boyko E.R. Effect of a Succinate-Containing Drug on the Blood Leukocyte Composition in Rats at Rest and During a Weight-Loaded Forced Swimming Test. Journal of Medical and Biological Research, 2021, vol. 9, no. 2, pp. 182–191. DOI: 10.37482/2687-1491-Z056

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Objective. Dibornol-HES, a water-soluble drug based on the derivative of 2,6-diisobornyl-4-methylphenol Dibornol conjugated with hydroxyethyl starch, can reduce the occurrence and severity of arrhythmias by preventive intravenous administration, but it is unknown whether the drug could reduce the myocardial arrhythmogenicity once ischemia has developed at the developed ischemia.Materials and methods. In the model of acute ischemia / reperfusion of the rabbit heart, the effect of Dibornol-HEC (80 mg/kg body weight of the animal) on the electrophysiological indices characterizing myocardial arrhythmogenicity (global and border dispersion of repolarization) was studied during the restoration of blood flow. In the model of acute ischemia / reperfusion with 64 unipolar epicardial leads, the activation-recovery intervals were measured and global and border dispersion of repolarization in the native rabbits (control group, n = 9) and in the rabbits treated by Dibornol-HES (on the 25th minute of occlusion, the experimental group, n = 6).Results. The introduction of Dibornol-HES did not lead to a change in the electrocardiographic parameters of rabbits. By the 30th minute of the coronary occlusion on the ECG in the animals of the control and the experimental groups, the intervals RR, QT, QTc were shortened (p < 0.05). In the animals of both groups by the 30th minute of coronary occlusion, the global dispersion of repolarization increased (p < 0.05), the boundary dispersion of repolarization also increased (p < 0.05), due to the decrease in the duration of the activation-recovery intervals in the ischemic zone (p < 0.05). During the 30-minute reperfusion the magnitude of the global dispersion of repolarization did not change in animals of the both groups, and the magnitude of the border dispersion of repolarization in the control rabbits decreased (p < 0.05), while in the rabbits treated by Dibornol-HES the border dispersion of repolarization did not changed.Conclusion. In rabbits of the experimental group, the values of the global and border dispersions of repolarization did not differ from those of the animals in the control group. Therefore, the administration to Dibornol-HES just prior to reperfusion does not lead to the decrease in the dispersion of repolarization increased as a result of acute ischemic myocardial damage.