The article studies the selection of enzyme preparations of amilolitichesky action intended for receiving a starchy mash with the increased content of solids after release of gluten from it are conducted. Also comparative characteristic of the main indicators of quality of the distiller's beer received on estimated and classical technology was carried out, varying dosages of osakharivayushchy fermental medicines from 2 to 8 units of GLA/g of conditional starch. Process of a sbrazhivaniye of the concentrated mash depending on a dosage of the osakharivayushchy fermental medicine Biozim 800 L is studied.
The xylanase enzyme with a specific activity of 5676.4 units/mg of protein and a purification degree of 53.1 was isolated and purified from the complex enzyme preparation Brusiem BGX. The enzyme was isolated by ethanol precipitation, and purification was performed by gel filtration on sefadex G-25 and G-150 and ion exchange chromatography on DEAE-cellulose. The effect of temperature and pH on the activity and stability of the enzyme in the temperature range of 30-70 °C and pH 4.0-7.0 was studied. The optimum action of xylanase is a pH of 5.5 and a temperature of 50 °C. The enzyme has a sufficiently high thermal and acid stability, hydrolyzes non-starchy polysaccharides containing (1,4) - β-D-xyloside bonds. In this regard, its use in biotechnology for hydrolysis of non-starchy polysaccharides of grain in the brewing and in the production of ethanol is prospectively.
The article studied yeast composition isolated from the separation of the mash, bran separated in the grinding step, as well as a protein supplement obtained by mixing these two products. Crude fat content ranges from 4,7 to 6,2%, crude protein content is 38%, which is 20% lower than in the fodder yeast, but 14% higher than that of the bran. It is also found that the resulting protein product enriched in micro- and macro-elements, as well as readily hydrolyzable and water soluble carbohydrates. crude fiber content is 5,5%, which is almost 2,5 times lower than in the bran. The composition of the feed protein supplement obtained with the introduction at the stage of aqueous suspension of wheat powdery cellulolytic enzyme preparation Viskolaza 150 L and without it. The enzyme preparation was added in the step of preparing aqueous suspensions and wheat powdery dosage of 0,01% by weight of the feedstock. protein feed additives obtained with and without addition of cellulolytic enzyme preparation of high quality crude protein content and protein. Thus, the content of crude protein in the protein with the introduction of an additive in an aqueous suspension of powdery Viskolazy 150 L was 37% whereas 34% without the enzyme preparation. The amount of soluble polysaccharides and the mass fraction of ash were practically at the same level of from 2,4 to 5% and from 5,5 to 7,0%, respectively. fiber content of the protein supplement using Viskolazy 150 L was 4,2-6,1%, which is 2,5 times lower than in the protein supplement obtained without enzyme preparation.
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