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N. Jekster'erno-konstitucional'nye osobennosti korov simmental'skoj porody razlichnyh genotipov v uslovijah promyshlennoj tehnologii proizvodstva moloka / T.N. Tishkina, A.A. Vel'matov, A.P. Vel'matov // Resursosberegajushhie jekologicheski bezopasnye tehnologii proizvodstva i pererabotki sel'skohozjajstvennoj produkcii.-Saransk, 2016.-S. 69-73. 11. Grupp, T. The Crossbreeding Type for Dairy Breeds / T. Grupp // Fleckviehworld.

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Reproductive disorders, presumably caused by Chlamydia abortus, are common among the ovine population of the Mari El Republic, Russia. C. abortus infection was determined by serologic testing or isolation and detection of the organism by PCR and direct immunofluorescence in tissue samples. Rams, ewes, and lambs (10 individuals each) were randomly chosen for serological testing by the complement fixation test and 7 of 30 (23%) animals tested were positive. Tissue samples were collected from ewes and aborted fetuses for isolation by inoculating chicken embryo yolk sacs (n = 41). The same samples were analyzed by PCR using commercial and in-house PCR kits and by direct immunofluorescence. C. abortus was detected in 58.5% of samples using PCR and in 60.9% of the samples by direct immunofluorescence. Five Chlamydia isolates were cultured in egg yolk sacs and adapted for growth in cell cultures. Phylogenetic analysis showed no substantial difference between Russian isolates and those from other parts of the world. The results of the study further demonstrate the usefulness of PCR for detection of C. abortus as a faster, simpler, and more reliable approach in comparison to culturing the organism and underscoring the necessity of screening for chlamydiosis as a cause of ovine abortion.

In experiments on rats, the effect of valprazolamide, a new antiepileptic agent from the group of amide derivatives of valproic acid, on the brain content of neuroactive amino acids (GABA, glycine, glutamic and aspartic acids) was studied. Detection of amino acids was performed by gas chromatography-mass spectrometry. It was shown that a single intraperitoneal injection of valprazolamide (100 mg / kg) increases the level of GABA and the amide form of glutamic acid, reduces the levels of asparagine, glutamic and aspartic acids in the rat brain.