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Recombinant human MIS (rhMIS) produced in transfected Chinese hamster ovary cells has been purified by immunoaffinity chromatography. In the absence of reducing agents, 140 kD homodimer and several oligomers with molecular masses from 280 to 1000 kD are present. Homodimer, tetramer, and higher-molecular-weight rhMIS fractions reduced survival of tumor cells. For these experiments, FITC-labeled rhMIS was used for binding and endocytosis studies by flow cytometry. Flow cytometry performed on MIS-sensitive cancer cell lines demonstrated specific binding of rhMIS. The majority of rhMIS receptors have cytosolic localization. Thus, the level of MIS receptors on the cell membrane was proportional to the content of MIS-binding proteins in the whole cell and defines a level of receptor-mediated endocytosis. The immunopurified rhMIS caused significant growth inhibition of ovarian and prostate adenocarcinoma and melanoma human cell lines in inhibition assays.

International studies show a declining population in the Arctic. The main contribution to the negative dynamics of migration loss belongs to the Russian Federation. The article presents the results of our study of the migration situation in the Murmansk region, which is part of the Arctic zone of the Russian Federation. The statistical analysis of the migration movement demonstrates active migration processes in the region. However, despite the increased rates of arrival in the region, the negative dynamics of population decline due to migration loss persists, when the number of people who drop out prevails over the number of arrivals. These data are indicators of a weak settled population in the Murmansk region and social unattractiveness of the region. We also supplemented the statistical analysis of the migration situation with the results of a sociological study of migration behavior, migration attitudes and migration motives of the residents of the Murmansk region. We found that 16% of respondents showed a positive migration attitude, 52% of respondents showed a negative migration attitude, and the share of “potential migrants” was 26%. The key reasons for migration from the North are unfavorable climatic conditions and the unfavorable socio-economic situation in the region. The social portrait of respondents with a positive migration attitude is described by the following socio-demographic variables. Gender - mostly female. Age - from 16 to 66 years (average value was 35 years). The level of education - secondary vocational (or specialized secondary) - 37%, and higher education - 26%. Duration of residence in the Murmansk region - from birth or almost all his life - 71%. Unemployed and employed - demonstrated a positive migration attitude equally. The motivational factors that constrain migration are own housing, employment, job satisfaction, wages and social guarantees, family-related and regional (arctic) identity.

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The article presents a program of a planned scientific experiment on a comprehensive study of the energy efficiency of the enclosing structures of a wooden low-rise building. The main focus of the study is on the construction of the wooden walls. Experimental buildings implemented in two constructive and technological solutions in different climatic conditions (climatic regions of the Republic of Karelia and the Murmansk region) are considered as objects of research. The review of normative methods of field studies of thermophysical properties of enclosing structures is carried out. An experimental research method is proposed by means of a system for monitoring temperature and humidity parameters, developed and installed in the layers of heterogeneous structures. As a result, a program of an experiment for a comprehensive study in a stationary mode of the internal microclimate of a building was formed.