To find out exactly how sowing density and sowing pattern affect soybean grain yield, quality and its components in non-traditional soybean growing regions, such as Germany, two field trials have been conducted at two locations in Bavaria in 2016 and 2017. The experiments were carried out with four sowing densities (30, 50, 70, 90 seeds/m<sup>2</sup>) and four cultivars from different maturity groups (Viola 000, Lissabon 000, ES Mentor 00, Orion 00) as randomized complete block design with four replicates. Almost all evaluated traits varied significantly depending on year × location. There was no interaction between the main factors (cultivar × sowing density). The results revealed that grain yield and height of the first pod increased with increase of sowing density. The number of pods per plant and branching decreased with increasing sowing density. At higher sowing densities at flowering leaf area index was significantly higher than at lower sowing densities. The cv. ES Mentor (00) with 70 seeds/m<sup>2</sup> has proved to be a suitable cultivar in terms of yield and quality in southern Germany (> 3.6 t/ha grain yield and 40% protein).
The cultivation area of soybean (Glycine max (L.) Merr) is increasing in Germany as a way to ensure self-sufficiency through its use as feed and food. However, climatic conditions needed for soybean cultivation are not appropriate in all parts of the country. The objective of this study was to determine the influence of solar radiation, temperature, and precipitation on soybean seed productivity and quality in central and south Germany. A multi-factorial field trial was carried out with three replicates at four locations in 2016 and five locations in 2017, testing 13 soybean varieties from the maturity groups MG 00 and MG 000. Considering all the tested factors, “variety” was highly significant concerning protein content (Ø 41.1% dry matter (DM)) and oil content (Ø 19.1% in DM), but not seed yield (Ø 40.5 dt ha−1).The broad sense heritability of protein content was H2 = 0.80 and of oil content H2 = 0.7. Protein and oil content were significantly negatively correlated (r = −0.82). Seed yield was significantly positively correlated with solar radiation (r = 0.32) and precipitation (r = 0.33), but significantly negatively with Crop Heat Units (CHU) (r = −0.42). Over both experimental years, varieties from maturity group MG 00 were less significantly correlated with the tested environmental factors than varieties from maturity group MG 000. None of the environmental factors tested significantly increased the protein or oil content of soybean. In growing areas with heat periods during ripening, protein content tended to be higher than in cooler areas; in areas with high solar radiation during flowering, protein content tended to be reduced.
Soybean (Glycine max L. Merrill) is one of the most important crops worldwide. In several European countries such as Germany, soybean cultivation is not traditional and as such remains to be optimized. One option to increase soybean production is to adapt and improve the seeding system. To investigate the effects of different seeding systems on seed yield, seed protein, and oil content, plant characteristics were assessed using a two-factorial (two seeding systems and four cultivars) field trial over two years (2017–2018) at two locations in southeast Germany. The seeding systems were drill (row spacing 14 cm) and precision seeding (row spacing 28 cm), and the cultivars were Viola 000, Lissabon 000, ES Mentor 00, and Orion 00. Depending on the seeding system, a seed yield of 3.8 t ha−1 dry matter (DM), 40.9% protein content (DM), and 18.8% oil content (DM) was achieved by drilling, and 3.6 t ha−1 yield (DM), 40.1% protein content (DM), and 19.1% oil content (DM) with precision seeding (average across four cultivars, two locations, and two years). No significant effects of the seeding system on all seed and plant characteristics were observed. As drilling and precision seeding did not affect the seed yield, seed protein, oil contents, and plant characteristics of soybean in this study, farmers are able to choose the seeding system which fits best into their farms and is economically most viable.
Relevance. One of the most common diseases of potatoes and other nightshade family species is late blight caused by a pathogenic oomycete of the Phytophthora infestans (Mont.) de Bary. At least 100 species of phytophthora have been described in nature, affecting a wide range of plant species. The phytophthora population is heterogeneous and is represented by races, as well as different types of mating. This leads to a rapid adaptation of the pathogen and the emergence of new, more aggressive, and resistant races. Phytophthora is a parasite, the damage from which cannot be avoided within the organic farming framework. Therefore, it is particularly important to know the pathogenesis and racial composition of phytophthora in each individual region of Solanaceae cultivation.Research methodology. Differentiation and collection of material from the natural population were carried out using potato varieties with known R-genes in the genome. Isolation and introduction into the culture were carried out from leaves with the dampening chambers method, followed by cultivation on nutrient media. The pathogen was identified by microscopic analysis. Culture filtrates were obtained on the liquid nutritious medium, followed by liquid filtration and autoclaving. Phytotoxic activity was determined by the effect on the seedlings of the nightshade, grass, and pea families by the standard method. Molecular genetic analysis of the isolates was carried out by ISSR analysis; the primer, amplification mixture, and temperature profile of the reaction were selected according to the literature data; the calculation of genetic characteristics was carried out using POPGENE software packages.Results. Samples of seven Phytophthora infestans isolates were collected and introduced into culture. As a result of in vitro cultivation, morphological differences were revealed, expressed in the structure and color of the mycelium, the shape of the colonies, the nature of sporulation, the color of the reverse, and the medium under the colonies. The genetic differences of the natural phytophthora material introduced into the culture, collected from potato varieties with single resistance genes (R1, R3, R4), were revealed. Differences in the phytotoxic activity of the studied isolates' cultural filtrates were revealed. The isolated isolates demonstrate differentiation at the phenotypic, genetic and physiological levels, which allows us to speak about their belonging to races.
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