Carbapenem-nonsusceptible (Carba-NS) Acinetobacter baumannii has emerged as an important cause of nosocomial infections. In the present study, we characterized 91 Carba-NS A. baumannii isolates collected from patients of surgical departments and intensive care units at three hospitals in Moscow in 2012-2015. Multilocus sequence typing (MLST) using the Oxford (Oxf) scheme identified 16 sequence types (STs) of three clonal complexes (CCs), including CC92 (67%), CC109 (1%), CC944 (29%), and the singleton ST1100 (3%). CC944 was composed of ST944 (n = 16) and two of its newly described single locus variants ST1103 (n = 3) and ST1104 (n = 7); all the three STs were identical to the Pasteur (Pas) MLST scheme ST78. All CC944/ST78 isolates were bla positive and all but one isolate harbored a bla gene. ST944 was the only ST found in each of the three study hospitals. Biofilm growth capacity was similar among Carba-NS and nonclonal carbapenem-susceptible isolates. Our data demonstrate the predominance of two clonal lineages among Carba-NS A. baumannii. One of these, the uncommon bla/bla-positive clone of CC944/ST78, seems to be endemic in Russia.

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Хроническая инфекция нижних дыхательных путей является ключевым признаком у больных муковисцидозом (МВ). Она является ведущим фактором, определяющим тяжесть клинического течения и прогноз заболевания. При изучении микрофлоры нижних дыхательных путей различ-ных возрастных групп детей, больных МВ, исследователями различных стран установлено, что основными возбудителями инфекции легких у больных МВ являются Р. aeruginosa, S. aureus и H. influenzae [1]. Показано, что в первые годы жизни у больных МВ доминирует золотистый стафилококк, а затем основным возбудителем становится синегнойная палочка [2, 3]. В последние годы было конста-С.В. ПОЛИКАРПОВА 1 , к.м.н., Е.И. КОНДРАТЬЕВА 2 , д.м.н., профессор, Л.А. ШАБАЛОВА 2 , к.м.н., Н.В. ПИВКИНА 1 , С.В. ЖИЛИНА 1 , А.Ю. ВОРОНКОВА 2 , В.Д. ШЕРМАН 2 , к.м.н., В.С. НИКОНОВА 2 , Н.И. КАПРАНОВ 2 , д.м.н., профессор, Н.Ю. КАШИРСКАЯ 2 , С.Ю. СЕМЫКИН 3 , Е.Л. АМЕЛИНА 4 , С.А. КРАСОВСКИЙ 4 1 Городская клиническая больница №15 им. О.М. Филатова Департамента здравоохранения г. Москвы 2 Медико-генетический научный центр, Москва

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Stenotrophomonas maltophilia is a common opportunistic microorganism and an important respiratory pathogen in cystic fibrosis (CF). The aim of this study was to determine antimicrobial resistance phenotypes, sequence-types (ST) and genetic determinants of antibiotic resistance in S. maltophilia strains recovered from CF patients in Russia. S. maltophilia isolates recovered from 170 CF patients were analyzed. Minimum inhibitory concentrations of antibacterial agents were determined using Sensititre Gram Negative GNX2F plates and the results were interpreted according to Clinical and Laboratory Standards Institute (CLSI) criteria. Whole-genome sequencing (WGS) was performed on MGISEQ-2000 platform. SPAdes software, Galaxy, ResFinder, Integrall and PubMLST were used for analysis of WGS data. S. maltophilia strains were identified from 24/170 (14%) CF patients. In total, 25 isolates were detected, two strains were isolated from the same patient. The isolates belonged to 17 different STs, including 5 new STs; ST4 was the most prevalent ST. Resistance to ceftazidime was observed in 60% of strains, to ticarcillin-clavulanate - in 32%, to levofloxacin - in 24%, to trimethoprim/sulfamethoxazole - in 12% of strains. All isolates were susceptible to minocycline. All ST4 isolates were resistant or intermediate to ceftazidime and ticarcillin-clavulanate. In two isolates, the sul1 gene was detected. In one isolate, sul1 was part of a class 1 integron. The detected integron also contained the blaGES-7 and aac(6’)-Ib-cr genes. The ST4 sequence-type was the most prevalent ST among S. maltophilia strains recovered from CF patients in Russia. Antibiotic resistance genes, including sul1, blaGES-7, aac(6’)-Ib-cr, were detected in single strains.