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Aim. To determine content of mononuclear ells (MNC) in peripheral blood of patients with coronary heart disease (CHD) and factors responsible for their functional state and cytokine production. Materials and methods. Concentration of proteinkinases JNK1/2, ERK1/2, MAPK38, AKT1, JAK2, FAK, AMPK, p70S6K, STAT3, STAT5B and STAT6 was determined in MNC lysate by immune-enzyme assay. Interleukin 1β, 2, 4 and y-interferon levels were measured in blood sera. Results. In patients with angina of effort and unstable angina, the JNK level was 59,8% and 53,1% higher than the normal one respectively (р=0,013) and (р=0,012). The level of the nuclear transcription factor was 26,9% (р=0,015) and 27,9% (р=0,017), JAK2 31,5% (р=0,022) and 48,6% (р=0,018), STAT3 49,6% (р=0,025) and 55,3% (р=0,02), STAT5B 21,5% (р=0,018) and 30,2% (р=0,011) lower. These changes were associated with a 13,1% (р=0,047) and 51,4% (р=0,019) rise in the STAT6 level, 30,1% (р=0,025) and 79,4% (р=0,003) FAK level, 7,6% (р=0,09) and 15,2% (р=0,039) АКТ1 level, 65,3 (р=0,02) and 76,2% (р=0,017) p70S6K level. Conclusion. Results of the study suggest persistent pro-inflammatory activation of whole blood cells in CHD patients due to enhanced levels of IL-1 and IL-2, components of the MAPK/SAPK signal pathway in MNC and decreased STAT3 level determining cell sensitivity to IL-10. The elevated intracellular level of ERK and JNK us responsible for high responsiveness of MNC to pro-inflammatory cytokines.

The study discusses the effect on the functional state of the whole blood cells of low-intensity microwave radiation. On the model of intercellular interactions in the conditions of spontaneous cell activity and by stimulating cells complex mitogen, the influence of microwave radiation 1000 MHz on production by whole blood cells interleukins: IL-1β, 2, 6, 8, 10, 12, 13, 17A, TNF-alpha, IFN-g, RAIL-1, and soluble forms of toll-like receptor 1, 2, 4, 6 types was studied. The research results show that a single 45 minutes of exposure of microwave radiation of 0.05 PPM microwatt/cm2 has the ability to enhance mitogen-stimulated production of RAIL-1 by 38,7% (p=0.03), to reduce the induced production of IL-1β by 26,3% (p=0,037), IL-8 by 56,2% (p=0.022) and to increase oppressed segment complex mitogen production of IL-10 by 27,8% (p=0,041). The microwave radiation increased spontaneous and mitogen-stimulated expression of TLR, especially TLR1 during the initial low level.

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The concentration of cytokines of the family IL-10 (IL-10, IL-22, IL-24), interferon-gamma, soluble forms of co-stimulatory molecules CD28, CD80, CD152, as well as NO was investigated in patients with coronary heart disease in interstitial fluid. In addition, the level of caspase-1, inducible and endothelial forms of NO synthasis (eNOS, iNOS), protein kinase AKT1 and AMPK and the total antioxidant activity from the cell culture supernantat were evaluatedin the agranulocytic patients. In the current study (in vitro) the impact on production of these mediators of low-intensity microwave radiation frequency of 1000 MHz, generated by an apparatus of low-intensity physiotherapy "Aquaton" was evaluated. Material of this study was whole venous blood of patients with angina pectoris and acute coronary syndrome. The analysis of the results revealed a reduced level of NO, IL-24, as well as increased production of IL-10, IL-22 and the concentration of soluble forms of CD28, CD80, CD152 in patients with coronary artery disease in the interstitial fluid in comparison with practically healthy persons. At a single irradiation of cell cultures whole blood by low-intensity microwave radiation frequency of 1000 MHz in patients with angina pectoris and acute coronary syndrome there were an increasing of NO production by 20,6 and 25,5‰, IL-24 by 18,6 and 33,6‰, and a reduction in intracellular caspase-1 by 32,5 and 25,8‰, as well as increasing of the levels of IL-10 by 3,5 and 3,1‰, IL-22 by 28,7 and 26,5‰ and reduction of the levels of CD28 by 3,6 and 3,9‰, CD80 6,8 and 5,7‰, CD152 by 9,4 and 11,3‰, respectively. In addition, in terms of irradiation there is an increase in interstitial fluid levels of antioxidants by 37,1 and 49,2‰.