Plum pox virus (PPV) is the causal agent of sharka, the most detrimental virus disease of stone fruit trees worldwide. PPV isolates have been assigned into seven distinct strains, of which PPV-C regroups the genetically distinct isolates detected in several European countries on cherry hosts. Here, three complete and several partial genomic sequences of PPV isolates from sour cherry trees in the Volga River basin of Russia have been determined. The comparison of complete genome sequences has shown that the nucleotide identity values with other PPV isolates reached only 77.5 to 83.5%. Phylogenetic analyses clearly assigned the RU-17sc, RU-18sc, and RU-30sc isolates from cherry to a distinct cluster, most closely related to PPV-C and, to a lesser extent, PPV-W. Based on their natural infection of sour cherry trees and genomic characterization, the PPV isolates reported here represent a new strain of PPV, for which the name PPV-CR (Cherry Russia) is proposed. The unique amino acids conserved among PPV-CR and PPV-C cherry-infecting isolates (75 in total) are mostly distributed within the central part of P1, NIa, and the N terminus of the coat protein (CP), making them potential candidates for genetic determinants of the ability to infect cherry species or of adaptation to these hosts. The variability observed within 14 PPV-CR isolates analyzed in this study (0 to 2.6% nucleotide divergence in partial CP sequences) and the identification of these isolates in different localities and cultivation conditions suggest the efficient establishment and competitiveness of the PPV-CR in the environment. A specific primer pair has been developed, allowing the specific reverse-transcription polymerase chain reaction detection of PPV-CR isolates.
Томат – важнейшая овощная культура с ежегодным увеличением объемов его производства во всем мире. В Российской Федерации последние несколько лет активно развиваются предприятия защищенного грунта, специализирующиеся на производстве томатов. Вместе с тем растения томата поражают более 200 различных вредителей и болезней. Возбудители вирусных болезней растений – важный ограничивающий фактор для многих отраслей растениеводства, в том числе овощеводства. В последние годы в европейских странах производство томатов в открытом и защищенном грунте пострадало от серьезных потерь, вызванных, главным образом, вирусными фитопатогенами. В статье представлен обзор трех наиболее опасных вирусов, возбудителей болезней томатов – коричневой морщинистости плодов томата, мозаики пепино, пятнистого увядания томата. Эти вирусы неоднократно были выявлены в целом ряде стран практически на всех континентах и вызвали значительные экономические потери в странах своего распространения. Ввиду очень быстрого распространения и обнаружения опасных вирусов томата в ряде стран, занимающихся производством и дальнейшим экспортом семян и плодов томатов, Федеральная служба по ветеринарному и фитосанитарному надзору Российской Федерации (Россельхознадзор) с 27 июля 2020 года ввела в качестве временной карантинной фитосанитарной меры требование об отсутствии этих вирусов в семенах, посадочном материале и плодах растений-хозяев при их ввозе и перемещении по территории Российской Федерации. Результаты анализов фитосанитарного риска, проведенных в ФГБУ «ВНИИКР» в 2020 году, показали, что вирусы коричневой морщинистости плодов томата, мозаики пепино и пятнистого увядания томата соответствуют критериям карантинных для Российской Федерации организмов, вирусы способны проникнуть на территорию страны с подкарантинной продукцией, распространиться и нанести существенный ущерб развитию сельского хозяйства и экономической деятельности страны. Tomato is the most important vegetable crop with an annual increase in its production worldwide. In the Russian Federation, greenhouse industry specializing in the production of tomatoes have been actively developing over the past few years. At the same time, tomato plants affect more than 200 different pests and diseases. Pathogens of viral diseases of plants are an important limiting factor for many branches of crop production, including vegetable growing. In recent years, in European countries, the production of tomatoes in open field and greenhouses has suffered from serious losses caused mainly by viral phytopathogens. The article presents an overview of the three most dangerous viruses, pathogens of tomato diseases – tomato brown rugose fruit virus (ToBRFV), pepino mosaic virus (PepMV) and tomato spotted wilt virus (TSWV). These viruses have been repeatedly detected in a number of countries on almost all continents and have caused significant economic losses in the countries of their distribution. In view of the very rapid spread and detection of dangerous tomato viruses in a number of countries engaged in the production and further export of tomato seeds and fruits, Rosselkhoznadzor, from July 27, 2020, introduced as a temporary quarantine phytosanitary measure the requirement that these viruses are not present in seeds, planting material and fruits of host plants when they are imported and moved through the territory of the Russian Federation. The results of the phytosanitary risk analyses conducted at the Federal State Budgetary Institution «VNIIKR» in 2020 showed that ToBRFV, PepMV and TSWV meet the criteria of quarantine organisms for the Russian Federation, viruses are able to enter the territory of the country with quarantined products, spread and cause significant damage to the development of agriculture and economic activity of the country.
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