The study aimed to reveal the difficulties of activating science laboratories in Palestinian and Jordanian secondary schools from the viewpoint of biology teachers in Jordanian public schools, in light of gender variables and years of experience. The study was conducted on a sample of Palestinian and Jordanian secondary school teachers affiliated with the Jordanian Ministry of Education. The study sample numbered (371) male and female teachers. During the second semester of the academic year (2018/2019). The questionnaire was developed in its final form consisting of (42) paragraphs distributed into (5) areas, and from the recommendations of the study, equipping school laboratories and providing the tools and materials necessary for experiments in the school laboratory in sufficient quantities, and the necessity of reviewing the manual of activities and practical experiments in science courses in general and biology in particular ,in term of specialization and amending it in proportion to the time of the prescribed quota for  biology.