The effect of different concentrations (5 and 7%) as powder of date palm pith on obese rats was evaluated. Twenty four male albino rats weighting 150 ±10 g were used in this study and divided into 4 groups, each group contained 6 rats. Rats were treated by high fat diet (20% animal fat) to induce obesity. Glucose, serum liver function (GOT GPT and ALP), lipids profile (TG, TC, LDL-c, HDL-c, VIDL-c, AI) and kidney function (uric acid, urea and creatinine)and serum protein fraction were determined. Results showed that the lower ALT, AST and ALP liver enzyme of treated group recorded for group fed on 7% date palm pith with significant differences. The lowest cholesterol and triglycerides, LDL-c and VLDL-c, uric acid, urea and creatinine values recorded for 7 % date palm pith with significant (p≤0.05) differences, while the highest (HDL-c) levels recorded for group fed on 7% mixture. As conclusion, obese rats treated with 5and 7 % powder of date palm had improved lipid profile, liver, kidney functions and glucose level. Therefore, date palm pith powder could be used in our beverages and daily level dishes, besides the fact that it has so many other health benefits.