The effects of the addition of Ceratobasidum stevensii B6 and its growth on the soil microflora at a continuously cropped watermelon (Citrullus lanatus) site in China. Acta Ecologica Sinica,2012,32(4) :1185鄄1192.
Serious obstacles exist for the continuous cropping of watermelon (Citrullus lanatus). It has previously been shown that following application of living Ceratobasidum stevensii B6 mycelia, the quantity of Fusarium oxysporum (FO) in continuously cropped soil at the harvest stage decreased by 29. 9% , while the total yield and soluble sugar content of the fruit increased by 103. 8% and 35. 1% compared with the control. Here we further discuss the mechanisms by which B6 may alleviate watermelon cropping problems. We developed a real鄄time polymerase chain reaction (PCR) assay to detect and quantify B6 in soil, and monitored the dynamics of the FO population in the soil. We also evaluated the effects of different fermentation components on the soil microflora, using Denatured Gradient Gel Electrophoresis (DGGE). B6鄄 specific DNA primers were designed based on the internal transcribed spacer (ITS) sequence. Amplification of B6 DNA
The rapid development of industrialization, urban鄄rural integration and agricultural activities are raising pollutions in China, especially pollutants of heavy metals in farmland soil. Effect of heavy metal pollutants on soil arthropods was an important part of their risk assessment and environmental impact monitoring. Based on the methods of National Soil Environmental Quality Standard of China (GB 15618-1995) , Cd (0.3 mg / kg and 0.6 mg / kg) , Pb (125 mg / kg and 250 mg / kg) and As (30 mg / kg and 60 mg / kg) were added to upland of red soil respectively, to evaluate their effects on the composition and diversity of soil microarthropods under field conditions. Eleven taxonomic groups of soil microarthropods were observed and Folsomia, Onychiurus and Acarina were the dominant groups, accounting for up to 78% of the total soil microarthropods obtained. Pb and As pollution in high concentration levels significantly decreased the abundance of Collembola by 73.5%, 55.2% respectively, and Cd pollution in high concentration levels did not have significant effects on Collembola. As and Cd pollution in high concentration levels significantly decreased the abundance of Acarina by 74.3%, 36.4% respectively, and Pb pollution in high concentration levels did not have significant effects on Acarina. However, Pb, As and Cd showed no significant influences on soil biodiversity, as estimated by the Shannon and Simpson indices.
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