Gellan gum as a natural polysaccharide has good heat resistance, acid resistance and enzymes resistance. However, one of the drawbacks of gellan gum might be the lower mechanical strength. In this work, gellan gum scaffolds were mixed with poly(lactic-co-glycolic acid) (PLGA) microsphere in order to improve mechanical properties. The gellan gum scaffolds with various contents of PLGA microsphere were prepared for the regeneration of disc tissues. To evaluate the mechanical strength of hybrid structure of gellan gum and PLGA microsphere, compression strength of the fabricated scaffolds was measured. MTT analysis, SEM observation, histological evaluation and RT-PCR were performed to confirm the effect on the cell growth and extracellular matrix secretion. As a result, it showed the best cell proliferation and extracellular matrix secretion in gellan gum sponge containing 50% PLGA microspheres. In conclusion, this study confirmed that the hybrid structure of gellan gum and PLGA microspheres was found suitable in regeneration of the intervertebral disc.

Demineralized bone particle (DBP) is a biomaterial used widely in the field of tissue engineering. In this study, in order to study the effect of DBP/poly(lactic-co-glycolic acid) (PLGA) scaffold on disc regeneration in vivo environment, we prepared the porous DBP/PLGA hybrid scaffold. Disc defect was induced by removing the nucleus pulposus tissue after incision the annulus fibrosus tissue in half and scaffolds were transplanted. After 1, 2 and 3 months later, the extracted discs were confirmed by collagen synthesis and glycosaminoglycan (sGAG). We conducted histology (H&E, Safranin-O, Alcian blue, Type I Collagen, Type II Collagen). From the results, it was confirmed that collagen and sGAG content were high in DBP/PLGA scaffold, and the regeneration of intervertebral disc was possible.

For biomaterials for skin regeneration with minimized inflammatory response, high bioactivity and biocompatibility are highly required. Also, it should have a porous microstructure to improve cell adhesion and growth. In this study, we extracted a new collagen source from duck's feet which is by-product, and made the shape of sponges from duck's feet collagen (DC) to compare with DBP and SIS. To analyze physical and chemical property of the scaffold, SEM and FTIR were used. MTT assay was used to measure the attachment and proliferation of NIH/3T3 in the scaffolds. RT-PCR was used to evaluate the expression of proinflammatory cytokine. Also, 1,1-diphenyl-2-picrylhydrazyl (DPPH) was used to measure the ability of antioxidant activity. Overall, this study shows that DC scaffold is biocompatible and has good physical property. Additionally, DC scaffold shows the potential as wound healing biomaterials. 차세롬·정현기·김수영·김은영·송정은·박찬흠·권순용·강길선폴리머, 제39권 제3호, 2015년 져야 한다. 7-9 이러한 생체재료로 천연고분자는 뛰어난 생체 적합성으로 인하여 체내에 이식 후 염증 반응이 적으며 우수 한 생분해성 및 생체기능성을 가지고 있어 조직공학용 지지 체로 이상적이다. 10-14 천연재료 중 하나인 콜라겐은 세포와의 친화력이 매우 우수 하며 생체 내 거부반응이 적기 때문에 생체의료용 재료로 주 목 받고 있으며, 또한 지방 성분이 함유되어 있지 않고 수용 성 단백질로 이루어져 흡수가 잘 이루어지는 장점이 있다. 15-17 피부, 뼈, 연골 등을 구성하는 당 단백질, 당 지질로 구조적 으로 세포 부착이 가능하며, 산소전달 및 신축성을 가지는 특 징으로 화장품의 원료뿐만 아니라 약물전달, 상처치유, 화상 치유 지지체로 필름, 3D 등의 형태로 널리 응용되고 있다. 18-21 또한 다양한 사이토카인의 존재는 세포 점착이나 성장, 이동 등 세포의 기능적인 면에 관여하여 조직 재생이나 상처 치유 에 매우 유용하게 작용한다. 조직공학에서는 콜라겐을 주로 소, 돼지에서 추출하여 사 용한다. 22,23 동물유래 콜라겐은 낮은 항원성, 지혈효과, 세포 부착능력이 우수하지만 콜라겐추출 공정기간이 너무 길다는 단점을 가진다. 전 세계적으로 소, 돼지에서 광우병, 구제역 이 발병하여 안정성에 관한 문제가 고려되고 있어 새로운 콜 라겐 원에 대한 연구가 진행되고 있다. 이에 따라 본 실험에서는 소, 돼지에 비해 안정성이 우수 하고 추출공정이 짧고 경제적으로 공급가격이 낮으며 제 1형 콜라겐이 풍부하다고 알려진 축산부산물인 오리발(duck's feet collagen, DC)을 사용하여 콜라겐을 추출하였다. 돼지에서 추 출한 소장 점막하조직(small intestinal submucosa, SIS)과 소 에서 추출한 탈미네랄화된 골분(demineralized bone powder, DBP)을 이전 연구에서 스펀지 형태로 제작하였을 때 가장 좋 은 효과를 보인 2 wt% 함량으로 스펀지를 제작하여 비교하 였다. 24,25 상처치유를 촉진하고 상처면의 삼출액을 조절함으로써 증 발로 인한 수분 손실과 상처의 오염방지를 위해 3차원으로 제작하였으며, 손상부위를 지혈하기 위해 스펀지 형태로 피 부재생용 지지체로의 가능성을 평가하였다. 26-28

No abstract

It has been widely accepted that costal cartilage cells (CCs) have more excellent initial proliferation capacity than articular cartilage cells as well as the easiness for isolation and collection. This study demonstrated that CCs might be one of the substitutes for articular cartilage cells by tissue engineered cartilage. Poly(lactic-co-glycolic acid) (PLGA) has been extensively tested and used as scaffold material but it was limited by the low attachment of cells and the induction of inflammatory cells. Base on previous our studies, we confirmed demineralized bone particle (DBP) had the power of the reduction of inflammatory reaction and the stimulation proliferation of cells. We fabricated PLGA scaffold loaded with 10, 20, 40 and 80 wt% DBP and then tested the possibility of the regeneration of cartilage using CCs. Assays of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and scanning electron microscope (SEM) carried out to evaluate the attachment and proliferation of CCs in DBP/PLGA scaffolds. Glycosaminoglycan (sGAG) and collagen contents assay were conducted to confirm the effects of DBP on formation of extracellular matrix. This study demonstrated that DBP/PLGA scaffolds showed significant positive effects on cell growth and proliferation due to the vitality of DBP as well as the possibility of the application of CCs for tissue engineered cartilage.