We investigated the inhibitory effect of Canavalia gladiata water extract (CGW) on ethanol‐induced gastric inflammation, specifically on gastric acid secretion and gastric mucosal damage in Sprague‐Dawley (SD) rats. The possible mechanisms of CGW actions via ROS was also further determined for antioxidant effects. Rats were divided into four groups : saline(G1), gastric inflammation plus saline(G2), gastric inflammation plus Lansoprazole 30mg/kg b.w(G3), gastric inflammation plus CGW 250mg/kg b.w(G4), gastric inflammation plus CGW 500mg/kg b.w(G5). After oral administration of either saline, Lansoprazole or CGW for 7 days, acute gastric inflammation was induced by oral administration of 1mL of 70% ethanol and 0.15M HCl at groups G2,G3,G4 and G5. After 1h on ethanol administration, all animals were sacrificed. Gastric acid secretion and gastric mucosal injury were significantly decreased in G3 when compared to that of G2. And groups pretreated with CGW (G4, G5) caused a significant decrease in gastric acid secretion and gastric mucosal injury in a dose dependant manner. To examine the regulatory factors related inflammation, NF‐kB, COX‐1, COX‐2 and cytokines were measured by immuno‐histochemistry and ELISA. The expression of NF‐kB, COX‐1 and COX‐2 were clearly decreased in G3 and G5 compared with the G2. Additionally, cytokines was significantly decreased in G3 and G5. We next examined the levels of MDA and SOD activity related in antioxidative effects. Oral administration of CGW significantly decreased levels of MDA and SOD activity was dramatically increased. In conclusion, our data show that CGW has inhibitory effects on gastric inflammation induced by acute ethanol treatment in SD rat. Although the exact mechanism underlying these actions is unclear, the effects on acute gastric lesions suggest a multifactorial mechanism probably involving the antioxidant properties of CGW.

No abstract

In this study, the general components and minerals of fermented Curcuma longa L. (FC) by Aspergillus oryzae were examined as well as the hepatoprotective effects of FC on acute hepatotoxicity induced by a single dose of galactosamine (GalN, 650 mg/kg body weight (b.w.)). The FC was found to consist of 0.15% moisture, 4.68% crude fat, 4.35% crude protein, 6.92% crude fiber, and 6.83% crude ash. The P, Ca, and Mg levels in FC were also quantitatively analyzed. Male Sprague-Dawley rats were divided into six groups; nontreated control, GalN, 150 mg/kg b.w. of silymarin plus GalN, 30 mg/kg b.w. of FC plus GalN, 100 mg/kg b.w. of FC plus GalN, and 300 mg/kg b.w. of FC plus GalN. Pretreatment 300 mg/kg b.w. of FC during 14 days significantly decreased the increased in aspartate aminotransferase, alanine amino transferase, and triglyceride (TG) induced by GalN. Severe liver damage, hepatocellular necrosis, infiltration of inflammatory cells, and councilman body necrosis on histopathological liver tissues were observed in GalN treated rats. Administration of 300 mg/kg b.w. of FC significantly decreased the degree of live damage. These results suggest that FC displays hepatoprotective activity and FC was able to lower the TG levels in serum; thus, FC may serve as a useful material for health food and clinical conditions associated with liver disease.

No abstract

No abstract