1,2 and 1,3 Propanediol, Microbial Production Methods

Song, Yuanquan; Xu, Yun‐Zhen; Liu, Dehua

doi:10.1002/9780470054581.eib001

Cited by 4 publications

(11 citation statements)

References 103 publications

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“…Overexpression of 1,3-PDO oxidoreductase led to faster glycerol conversion and 1,3-PDO production. After 12-h conversion, it improves 1,3-PDO yield by 20.4%, and boosts product/substrate yield from 50.8 to 59.8% (mol/mol) (Song et al, 2010). Further investigations should be done to address if 3-HPA is occurring in E. coli SZ63 during 1,3-propanediol production and see the need to overexpress 1,3-PDO oxidoreductase.…”

Section: Reactor Fermentation With E Coli Sz63mentioning

confidence: 99%

Anaerobic and micro-aerobic 1,3-propanediol production by engineered Escherichia coli with dha genes from Klebsiella pneumoniae GLC29

Neto¹,

Briganti²,

Layton³

et al. 2017

Afr. J. Biotechnol.

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1,3-Propanediol (1,3-PDO) is a bifunctional molecule, and used in applications similar to those of ethylene glycol, propylene glycol, 1,3-butanediol and 1,4-butanediol. The use of glycerol as a feedstock is an alternative to reduce production costs for both 1,3-PDO and biodiesel, since biodiesel glycerol can be used for the production of 1,3-PDO by bacteria. Also, using metabolic engineering, it is possible to manipulate the metabolic routes and obtain high value products, reduce or eliminate the formation of undesirable byproducts. The aim of the study was to produce 1,3-propanediol in E. coli cloned with dha genes from Klebsiella pneumoniae GLC29. Six genes responsible for 1,3-PDO production in Klebsiella pneumoniae GLC29 were cloned. These genes were assembled in pSB1C3 as an expression vector: Genes dhaB1, dhaB2, dhaB3 and dhaT (pSB1C3dhaB123T), and another vector with genes dhaF and dhaG (pSB1C3dhaB123TFG) were derived using Gibson's Assembly technique. Escherichia coli TCS099 and SZ63 stains were used as hosts for 1,3-PDO production, and kept at -80°C for long-term storage. Glycerol was used as the sole or main carbon source in all experiments. Fermentations were performed in flasks in aerobic and anaerobic conditions using minimal media. Also, two stage fermentation (aerobic-anaerobic) was performed for 1,3-propanediol production. Only pSB1C3dhaB123TFG was able to produce high amounts of 1,3-PDO in shake flasks experiments, producing 2.5 g/L in micro-aerobic conditions, using E. coli TCS099 as host. Besides, E. coli SZ63 hosting pSB1C3dhaB123TFG was able to produce high amounts of 1,3-PDO, corresponding to 11.3 g/L of 1,3-PDO using a two-stage fermentation process using low concentration of vitamin B12 (1 mg/L). Plasmid pSB1C3dhaB123TFG shows potential for producing high amounts of 1,3-PDO, specially because of dhaF and dhaG, reaffirming the importance of this genes on 1,3-PDO production, especially with the addition of low amounts of vitamin B12, which is an expensive compound.

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Section: Reactor Fermentation With E Coli Sz63mentioning

confidence: 99%

Anaerobic and micro-aerobic 1,3-propanediol production by engineered Escherichia coli with dha genes from Klebsiella pneumoniae GLC29

Neto¹,

Briganti²,

Layton³

et al. 2017

Afr. J. Biotechnol.

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“…Gas sparging in animal cell culture is thus used judiciously. 53 53 A common feature of record-highdensity animal cell cultures is that they are not sparged at all, with O 2 instead supplied via the bioreactor headspace [112,113].…”

Section: Bioreactor Design Principles and Limitationsmentioning

confidence: 99%

“…Guidance on maximum sparge rate is usually <0.1 vvm [54,118], though the O 2 demand of most low-to medium-density cell cultures can usually be satisfied with much less. 55 For agitation, historical guidance has been to 55 0.01 vvm is common [53]. Additionally, copious surfactants for foaming and shear control are typically recommended to minimize cell damage in vigorously sparged cultures, e.g., Pluronic F-68 at >0.5 g/L [111].…”

Section: Bioreactor Design Principles and Limitationsmentioning

confidence: 99%

“…The equivalent limits for animal cell culture are~0.01 vvm and~100 W/m 3 . These constraints place animal cell culture in a significantly lower oxygen mass transfer regime than fermentation; upper limits of the O 2 mass transfer coefficient k L a are~800 h -1 in fermentation and~15 h -1 in cell culture [53]. Due to the animal cells' lower growth rate, the OTR achievable with such a low k L a is generally adequate to match OUR as cells multiply to low/medium density [54].…”

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confidence: 99%

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Scale-Up Economics for Cultured Meat: Techno-Economic Analysis and Due Diligence

Humbird¹

2020

Preprint

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“Cultured meat” technologies aim to replace conventional meat with analogous or alternative bioproducts from animal cell culture. Developers of these technologies claim their products, also known as “cell-based” or “cultivated” meat, will be safer and more environmentally friendly than conventional meat while offering improved farm-animal welfare. To these ends, Open Philanthropy commissioned this assessment of cultured meat’s potential to measurably displace the consumption of conventional meat.Recognizing that the scalability of any cultured-meat products must in turn depend on the scale and process intensity of animal cell production, this study draws on techno-economic analysis and due-diligence perspectives in industrial fermentation and upstream biopharmaceuticals to assess the extent to which animal cell culture could be scaled like a fermentation process.The analysis identifies a number of significant barriers to the scale-up of animal cell culture. Bioreactor design principles indicate a variety of issues associated with bulk cell growth in culture: Low growth rate, metabolic inefficiency, catabolite and CO2 inhibition, and bubble-induced cell damage will all limit practical bioreactor volume and attainable cell density. With existing bioreactor designs and animal cell lines, a significant engineering effort would be required to address even one of these issues.Economic challenges are further examined. Equipment and facilities with adequate microbial contamination safeguards are expected to have high capital costs. Suitable formulations of amino acids and protein growth factors are not currently produced at scales consistent with food production, and their projected costs at scale are likewise high. The replacement of amino-acid media with plant protein hydrolysates is discussed and requires further study.Capital- and operating-cost analyses of conceptual cell-mass production facilities indicate production economics that would likely preclude the affordability of their products as food. The analysis concludes that metabolic efficiency enhancements and the development of low-cost media from plant hydrolysates are both necessary but insufficient conditions for the measurable displacement of conventional meat by cultured meat.

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“…However, new applications are being studied for large volumes of biodiesel glycerol, since it can't be used in food and cosmetics industries without a cleaning and refining process [7]. Glycerol may be used as carbon source in bioprocesses, and several species among the genera Clostridium, Citrobacter, Klebsiella and Pseudomonas [5,[7][8][9][10][11][12][13] are being studied for the biotransformation of glycerol from biodiesel to specialty chemicals. One promising solution is the use of biodiesel glycerol to produce 1,3-propanediol (1, by Klebsiella pneumoniae and Clostridium butyricum [14,15].…”

Section: Introductionmentioning

confidence: 99%

Experimental Design For 1,3-Propanediol Biosynthesis by K. Pneumoniae GLC29 Using Glycerol

Neto¹,

Silva²,

Coelho³

et al. 2017

JABB

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Biodiesel glycerol can be used for the production of 1,3-propanediol by bacteria. In this study on the production of 1,3-propanediol by Klebsiella pneumoniae GLC29 was possible to optimize media culture the production of 1,3-propanediol using statistical experimental design techniques and response surface methodology, where 11 variables were tested and only 5 were found significant (glycerol, yeast extract, ammonium sulfate, vitamin B12 and fumarate). Subsequently, production of 1,3-propanediol was verified in 0,75 liter reactors, both in batch and exponential fedbatch. It was possible to achieve 23.6 g/l of 1,3-propanediol in batch cultures using pure glycerol, 27 g/l of 1,3-propanediol in batch cultures using biodiesel glycerol, and 29.9 g/l of 1,3-propanediol in exponential fed-batch using biodiesel glycerol.

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1,2 and 1,3 Propanediol, Microbial Production Methods

Cited by 4 publications

References 103 publications

Anaerobic and micro-aerobic 1,3-propanediol production by engineered Escherichia coli with dha genes from Klebsiella pneumoniae GLC29

Anaerobic and micro-aerobic 1,3-propanediol production by engineered Escherichia coli with dha genes from Klebsiella pneumoniae GLC29

Scale-Up Economics for Cultured Meat: Techno-Economic Analysis and Due Diligence

Experimental Design For 1,3-Propanediol Biosynthesis by K. Pneumoniae GLC29 Using Glycerol

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