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Srikwan, Sukamol; Hufford, Kristina M.; Eggert, Lori S.; Woodruff, David S.

doi:10.2306/scienceasia1513-1874.2002.28.093

ScienceAsia

2002

DOI: 10.2306/scienceasia1513-1874.2002.28.093

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Sukamol Srikwan¹,

Kristina M. Hufford²,

Lori S. Eggert³

et al.

Abstract: We describe the sequences of six primer pairs for the PCR amplification of nuclear microsatellite markers in the tree shrews, Tupaia glis and T. belangeri. Multilocus genotyping based on non-destructive DNA sampling of live-trapped animals reveals high allelic variability (A) and heterozygosity (H e ) at these loci. Such characteristics make these genetic markers ideal for linkage mapping and comparative genomics, and for studies of pedigree relationships, population structure, and population genetic variabili… Show more

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Cited by 4 publications

References 17 publications

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Limited effect of anthropogenic habitat fragmentation on molecular diversity in a rain forest skink, Gnypetoscincus queenslandiae

Sumner

Jessop

Paetkau³

et al. 2004

Molecular Ecology

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To examine the effects of recent habitat fragmentation, we assayed genetic diversity in a rain forest endemic lizard, the prickly forest skink (Gnypetoscincus queenslandiae), from seven forest fragments and five sites in continuous forest on the Atherton tableland of northeastern Queensland, Australia. The rain forest in this region was fragmented by logging and clearing for dairy farms in the early 1900s and most forest fragments studied have been isolated for 50-80 years or nine to 12 skink generations. We genotyped 411 individuals at nine microsatellite DNA loci and found fewer alleles per locus in prickly forest skinks from small rain forest fragments and a lower ratio of allele number to allele size range in forest fragments than in continuous forest, indicative of a decrease in effective population size. In contrast, and as expected for populations with small neighbourhood sizes, neither heterozygosity nor variance in allele size differed between fragments and sites in continuous forests. Considering measures of among population differentiation, there was no increase in FST among fragments and a significant isolation by distance pattern was identified across all 12 sites. However, the relationship between genetic (FST) and geographical distance was significantly stronger for continuous forest sites than for fragments, consistent with disruption of gene flow among the latter. The observed changes in genetic diversity within and among populations are small, but in the direction predicted by the theory of genetic erosion in recently fragmented populations. The results also illustrate the inherent difficulty in detecting genetic consequences of recent habitat fragmentation, even in genetically variable species, and especially when effective population size and dispersal rates are low.

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Limited effect of anthropogenic habitat fragmentation on molecular diversity in a rain forest skink, Gnypetoscincus queenslandiae

Sumner

Jessop

Paetkau³

et al. 2004

Molecular Ecology

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Isolation and characterization of polymorphic microsatellite loci in Bornean treeshrews (Tupaia spp.)

Munshi‐South

Wilkinson

2006

Molecular Ecology Notes

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In this study, we developed five microsatellite loci from an enriched genomic library constructed for the pygmy treeshrew ( Tupaia minor ), and adapted another two from a previous study on the common treeshrew ( Tupaia glis ), for use in studying mating and dispersal patterns in Bornean treeshrews. We screened 32 plain treeshrew ( Tupaia longipes ) and 54 large treeshrew ( Tupaia tana ) individuals at these loci. Polymorphism ranged from two to 13 alleles, and heterozygosity ranged from 0.29 to 0.88. These results indicate the general utility of these microsatellites for genetic analyses in other Tupaia spp.

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Analysis on Microsatellite Loci of Daphnia similoides sinensis and Rapid Development of Primer Based on Transcriptome Sequencing

Zhang

Deng

et al. 2019

Russ J Genet

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Cited by 4 publications

References 17 publications

Limited effect of anthropogenic habitat fragmentation on molecular diversity in a rain forest skink, Gnypetoscincus queenslandiae

Limited effect of anthropogenic habitat fragmentation on molecular diversity in a rain forest skink, Gnypetoscincus queenslandiae

Isolation and characterization of polymorphic microsatellite loci in Bornean treeshrews (Tupaia spp.)

Analysis on Microsatellite Loci of Daphnia similoides sinensis and Rapid Development of Primer Based on Transcriptome Sequencing

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